摘要
目的探讨丙型肝炎病毒(HCV)对胶原三股螺旋重复蛋白1(CTHRC1)表达的影响。方法 RT-PCR和Western blot检测JFH-1株HCV病毒感染的Huh7.5.1细胞及其对照细胞中CTHRC1 mRNA和蛋白的表达水平,酶联免疫吸附试验(ELISA)检测CTHRC1血清学含量。结果 HCV感染的Huh7.5.1细胞CTHRC1 mRNA和蛋白的表达水平较对照细胞高,与健康对照组相比,丙肝患者CHTRC1血清学水平显著升高(P<0.05)。结论 HCV能够在体内外上调CTHRC1的表达。
Objective To explore the effect of hepatitis C virus （HCV） on the expression of collagen triple helix repeat containing 1 （CTHRC1）. Methods mRNA and protein expression of CTHRC1 in Huh7.5.1 cells infect- ed by HCV JFH-1, the control cells were measured by RT-PCR and western blot, and serum levels of CTHRC1 were measured by enzyme-linked immunosorbent assay （ELISA）. Results Expression of CTHRC1 mRNA and protein was higher in Huh7.5.1 cells infected by HCV JFH-1 than in the control cells, and serum CTHRC1 levels was much higher in HCV patients than in healthy controls （P〈0.05）. Conclusion HCV can up-regulate the expression of CTHRC1 both in vivo and in vitro.
出处
《海南医学》
CAS
2013年第17期2497-2498,共2页
Hainan Medical Journal
基金
国家自然科学基金(编号:81101485)
国家临床重点专科专项经费(编号:2010305)
中国博士后特别资助基金(编号:201104485)
湖北省教育厅科学技术研究项目(编号:D20122404)
关键词
丙型肝炎病毒
胶原三股螺旋重复蛋白1
酶联免疫吸附试验
Hepatitis C virus
Collagen triple helix repeat containing 1
Enzyme-linked immunosorbent assay （ELISA）
