摘要
目的探讨全耐型铜绿假单胞菌(PRPA)与多耐型铜绿假单胞菌(MRPA)的代谢谱,为铜绿假单胞菌的耐药表型与代谢的研究和临床治疗提供依据。方法采用美国BD公司phoenix100全自动细菌鉴定、药敏仪NMIC、ID-4鉴定、药敏板对17株PRPA和23株MRPA进行鉴定,对其45种不同代谢指标进行统计,检验其结果差异是否有统计学意义。结果在45项代谢指标中L-焦谷氨酸-AMC代谢指标PRPA阳性率58.82%低于MRPA的100.00%(P<0.01);L-脯氨酸-AMC、Gamma-谷氨酰-NA代谢指标,PRPA阳性率分别为70.59%、52.94%显著高于MRPA的阳性率13.04%、4.35%,差异均有统计学意义(P<0.01)。结论 PRPA与MRPA 3种阳性代谢指标差异有统计学意义,说明铜绿假单胞菌在不同的环境条件下,产生不同的代谢。
OBJECTIVE To investigate the metabolite profiling of two resistant phenotypes., the pandrug-resistant Pseudomonas aeruginosa （PRPA）and multidrug-resistant Pseudomonas aeruginosa （MRPA）, so as to provide basis for the study of the drug resistant phenotypes and metabolism and guide the clinical treatment. METHODS Totally 17 strains of PRPA and 23 strains of MRPA of Pseudornonas aeruginosa were identified by adopting BDPs phoenixTM100, drug susceptibility NMIC, ID-4 , and the drug susceptibility plate the 45 metabolite indicators were statistically analyzed to see whether there was statistical difference. RESULTS Among the 45 metabolite indi- cators, the positive rate of the metabolite indicator LPYR was lower in PRPA （58.82%） than in the MRPA （100.00%） （P〈0.01）; the positive rates of the metabolite indicators LGGH and LPROB were higher in PRPA （70.59% , 52. 94%）than in the MRPA （13.04%, 4. 35%）, the difference was statistically significant （P〈 0.01）. CONCLUSION The difference in the three metabolite indicators between the PRPA and the MRPA is sig- nificant, indicating that the P. aeruginosa may produce different metabolites under different environment condi- tions.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2013年第15期3564-3565,3577,共3页
Chinese Journal of Nosocomiology
关键词
铜绿假单胞菌
代谢
耐药表型
Pseudomonas aeruginosa
Metabolite profiling
Resistant phenotype
