摘要
目的：了解浙江省湖州市中国人民解放军第98医院自临床分离的多重耐药铜绿假单胞菌(MRPA)中氨基糖苷类修饰酶(AMEs)基因存在状况。方法：在2003年9月至2004年10月间从临床分离30株MRPA,采用聚合酶链反应(PCR)技术检测9种AMEs基因[aac(3)-Ⅰ、aac(3)-Ⅱ、aac(3)-Ⅲ、aac(3)-Ⅳ、aac(6′)-Ⅰ、aac(6′)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ和aph(3′)-Ⅵ]。结果：30株MRPA中各种AMEs基因的阳性株数(阳性率)分别为aac(3)-Ⅱ 23株(76.7％)、aac(6′)-Ⅰ 1株(3.3％)、aac(6′)-Ⅱ 10株(33.3％)、ant(3″)-Ⅰ 16株(53.3％)、ant(2″)-Ⅰ 8株(26.7％),而aac(3)-Ⅰ、aac(3)-Ⅲ、aac(3)-Ⅳ和aph(3′)-Ⅵ均为阴性。基因总阳性率为86.7％(26／30)。结论：第98医院临床分离的多重耐药铜绿假单胞茵中AMEs基因携带率很高,至少存在5种AMEs基因,分别为aac(3)-Ⅱ、aac(6′)-Ⅰ、aac(6′)-Ⅱ、ant(3″)-Ⅰ和ant(2″)-Ⅰ。
Objective： To investigate the existed situation of the aminoglycoside modifying enzymes（AMEs） genes in multi-resistant Pseudomonas aeruginosa （MRPA） isolated from the People＇s Liberation Army 98th Hospital,Huzhou District,Zhejiang Province,China. Methods： Thirty strains of MRPA were isolated from hospitalized patients from Sep. 2003 to Oct, 2004. Nine kinds of AMEs genes of aac（3）- Ⅰ , aac（3）-Ⅱ, aac（3）-Ⅲ, aac（3）-Ⅳ, aac（6′）-Ⅰ , aac（6′）-Ⅱ, ant（3＂ ）- Ⅰ , ant（2＂ ）-Ⅰ , and aph（3＇）-Ⅵ were detected by polymerase chain reaction（PCR）. Results： In 30 strains of MRPA, the positive rates of genes of aac（3）-Ⅱ, aac（6′）-Ⅰ, aac（6′）-Ⅱ, ant（3＂ ）-Ⅰ and ant（2＂ ）-Ⅰ were 76.7%, 3.3%, 33.3%, 53.3% and 26.7%, respectively. The genes of aac（3）-Ⅰ, aac （3）- Ⅲ, aac（3）- Ⅳ, and aph（3′）-Ⅵ were all negative. The total positive rate of AMEs genes was 86.7%. Conclusions： The positive rates of AMEgenes are very high, and 5 kinds of genes, including aac（3）-Ⅱ, aac（6′）-Ⅰ , aac（6′）- Ⅱ, ant（3＂ ）-Ⅰ and ant（2＂ ）-Ⅰ in MRPA are identified.
出处
《温州医学院学报》
CAS
2005年第6期457-460,共4页
Journal of Wenzhou Medical College
基金
温州市科技局科研基金(Y2004A116)
关键词
多重耐药铜绿假单胞菌
氨基糖苷类修饰酶
基因
multi-resistant Pseudomonas aeruginosa
aminoglycoside modifying enzymes
gene
