摘要
目的 ：研究三七总皂苷（PNS）对脂多糖（LPS）诱导的大鼠心肌细胞肥大的保护作用。方法：体外培养新生1~2d的SD大鼠乳鼠心肌细胞48 h后,设正常对照组、脂多糖1mg/L模型组、脂多糖＋三七总皂苷12.5、25、50 mg/L组。考马斯亮蓝法测细胞的蛋白含量;计算机图像分析系统测量细胞体积;酶联免疫吸附实验（ELISA）检测细胞肿瘤坏死因子（TNF-α）的含量;以Fura-2/AM为荧光探针,采用Till阳离子测定系统,观测细胞内[Ca2＋]i瞬间变化。结果：与正常对照组相比,脂多糖1 mg/L可使心肌细胞蛋白含量明显增多,体积显著增大,释放的TNF-α含量显著增加,心肌细胞内[Ca2＋]i瞬间峰值增大。与脂多糖模型组相比,预加入12.5、25、50 mg/L的三七总皂苷均可抑制心肌细胞蛋白含量增多,体积增大,同时细胞产生的TNF-α含量明显降低,抑制了细胞内[Ca2＋]i瞬间变化幅度增大。结论：三七总皂苷对脂多糖诱导的大鼠乳鼠心肌细胞肥大有良好的保护作用,其机制可能与抑制TNF-α的产生,降低[Ca2＋]i有关。
Objective： The aims of our study was to investigate the protective effects and mechanism of lipopolysaccharide（LPS）-induced cardiac hypertrophy by Panax notoginseng saponins（PNS）.Method： 1 ~ 2d in vitro cultured neonatal SD rats neonatal rat cardiac myocytes after 48h,Myocardial cells of neonatal rats were cultured in vitro and were divided into normal control groups,LPS 1mg / L model group,LPS ＋ PNS 12.5,25,50 mg / L group.The total protein was measured by the Bradford Protein Assay Kit.The cardiomyocyte volume was assayed by computer photograph analysis;tumor necrosis factor（TNF-α） levels was determined by enzyme-linked immunosorbent assay（ELISA）;[Ca2 ＋]i transient was measured by Till image system by cell-loading Fura-2/AM.Result： Compared with normal control group,LPS 1mg/L significantly increased the total protein content and the size of myocardial cells,the release of TNF-α protein was significantly increased,and intracellular [Ca2 ＋]i transient peak increase（P ＆lt;0.01）.Compared with the LPS model group,preaccession 12.5,25,50mg/L of PNS can inhibit myocardial cell volume and total protein content increases,while cells of TNF-α were significantly reduced,and the cell viability increases,inhibit intracellular [Ca2 ＋]i transient peak increase.Conclusion ： PNS do has a good protective effect of cultured cardiac myocytes and its mechanism may be related to decreased release of TNF-α and inhibiting [Ca2 ＋]i.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第4期64-67,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
辽宁省科技计划项目(2009225010-40)
辽宁省教育厅创新团队项目(2009T064)
关键词
三七总皂苷
脂多糖
心肌肥大
肿瘤坏死因子α
钙离子浓度
Panax notoginseng saponins（三七总皂苷）
lipopolysaccharide
cardiac hypertrophy
TNF-α
[Ca2 ＋]i
