摘要
目的研究海洋生物口虾蛄乙酸乙酯提取物联合阿霉素或顺铂对人肝癌HepG2细胞株的增殖抑制效应,并定量分析其协同、相加或拮抗的作用。方法不同浓度的口虾蛄乙酸乙酯提取物联合阿霉素或顺铂处理肝癌HepG2细胞株24 h,用MTT法测定细胞的生长抑制作用,并用中效原理法、金氏修正公式分析药物的联合作用。结果口虾蛄乙酸乙酯提取物、阿霉素、顺铂单用或联合用药作用于肝癌HepG2细胞株,均能抑制细胞的增殖,呈现量-效依赖性。口虾蛄乙酸乙酯提取物与传统化疗药物的联合用药增加了传统化疗药物对肝癌细胞的毒性作用,呈现出低浓度拮抗,而高浓度协同的作用。结论海洋生物口虾蛄乙酸乙酯提取物作为一类新型、天然的抗肿瘤药物,可有效地抑制肝癌HepG2细胞的增殖。
Objective To study inhibitory effects on proliferation of human hepatoma line HepG2 by ethyl acetate extract of squilla oratoria （ESO） in combination with routine anticancer chemotherapeutic agents adriamycin or cisplation, and qunatatively analyze the additive or antagonist effects between ESO and the two drugs. Methods The HepG2 cells were treated with either ESO, adriamycin or cisplatin alone, or ESO/adriamycin, ESO/cisplatin in combination for 24 h. The growth inhibition was assayed with MTT method, and the synergistic effect of different drugs was evaluated with Median-effect principle, the Revised Jin＇s formula. Results The three drugs all exerted inhibitory effect on the growth of HepG2 cells in a dose-dependent manner, and the combination also decreased the proliferation of the cells. The combination with different chemotherapy agents increased cytotoxic effects on ceils, and low concentration showed a synergistic effect, but the high concentrations demonstrated the antagonistic effect. Conclusions Marine squilla oratoria as a new class of natural anticancer drug, can effectively inhibit the HepG2 cells proliferation.
出处
《中国医药生物技术》
2013年第5期344-348,共5页
Chinese Medicinal Biotechnology
基金
广东省教育厅重点学科建设基金(GX9404)
湛江市科技攻关项目(2009C3101017)
关键词
肝肿瘤
联合用药
中效原理
Liver neoplasms
Combination therapy
Median-effect principle
