摘要
[目的]制备胆固醇基普鲁兰多糖阿霉素自组装纳米粒(Self-assembled ADM-loaded cholesterol modified pullulan nanoparticles,ADM-CHSP-SAN)并考察其体外抗肿瘤活性。[方法]以胆固醇基普鲁兰多糖(cholesterol-modified pullulan,CHSP)为载体,采用透析法制备ADM-CHSP-SAN,并测定其形态、粒径、Zeta电位、包封率和载药量,采用MTT法研究其抑制U251肿瘤细胞的活性作用。[结果]ADM-CHSP-SAN外观呈圆形或类圆形,平均粒径为(112.8±1.02)nm,Zeta电位为(-27.2±0.246)mV,包封率和载药量分别为(67.14±1.21)%和(7.65±0.58)%;体外释药行为符合Higuchi方程;给药剂量大于25μg.mL-1时,ADM-CHSP-SAN抑制U251肿瘤细胞的活性作用明显优于阿霉素溶液剂(P<0.01)。[结论]将阿霉素制备成ADM-CHSP-SAN可有效提高药物的抗肿瘤活性。
[Objective] To prepare adriamycin self-assembled nanoparticles, and study the in vivo anti-tumor activity. [Methods]The self-assembled adri- amycin loaded cholesterol-modified pulhilan nanoparticles were prepared by dialysis and were characterized by morphology for particle size,Zeta potential, entrapment efl^ciency,dmg loading content.They were incubated with U251 cells to assess the inhibition ability of the self-assembled adriamycin-loaded cholesterol-modified pulhilan nanoparticles. [Results]The morphology of self-assembled adriamycin loaded cholesterol-modified pullulan nanoparticles was spherical. The mean particle size, Zeta potential, entrapment e^ciency and drag loading were （112.8＋1.02）nm,（-27.2＋0.246）mV,（67.14_＋1.21）% and （7.65＋0.58）%, respectively.The profiles of release were expressed well by Higuchi equation. When the dosages were 25p^g＇mL ＇ plus, the inhibiton ability against U251 was stronger than adriamycin solution（P〈0.01）.[Conclusion]The self-assembled adriamycin loaded cholesterol-modified pullulan nanoparticles exhibited more cycitoxic activity against U251 than adriamycin solution.
出处
《浙江中医药大学学报》
CAS
2013年第8期951-955,共5页
Journal of Zhejiang Chinese Medical University
基金
浙江中医药大学科研基金(2012ZY16)~~
关键词
阿霉素
胆固醇基普鲁兰多糖
自组装纳米粒
体外释放
抗肿瘤
adriamycin
cholesterol-modified pullulan
self-assembled nanoparticles
in vitro release
antitumor
