摘要
目的：建立RP—HPLC测定不同产地黄芩中黄芩苷含量的方法。方法：采用高效液相色谱法，AgilentTC—C18（4．6mmx250mm，5gm）色谱柱，以甲醇-0．4％磷酸（40：60）为流动相，流速1．0mL·min-1，检测波长278nm，柱温25℃。结果：黄芩苷在0．18-0．48gg（r=0．9999）范围内呈良好线性关系，平均回收率分别为98．56％（RSD为1．03％）。结论：所建立的方法重现性好、操作简便、结果准确可靠，为黄芩的质量评价和资源利用提供了实验依据。
Objective： To establish a method of baicalin in scutellaria baicalensis from different habitats with RP-HPLC determined. Methods： High performance liquid chromatography and chromatographic column of Agilent TC-C18（4.6mm x 250mm,tm） were used, with methanol-0.4% phos- phoric acid （40：60） as mobile phase, flow rate of 1.0mL. min-1, detection wavelength of 278nm and column temperature of 25℃. Results： Baicalin ranging from 0.18 to 0.48g （r=0.9999） presented a good linear relationship, and the average rate of recovery was 98.56% （RSD of 1.03%）. Conclu- sion： The established method of good reproducibility, simple and convenient operation, accurate and reliable results, provides the experimental basis for the quality evaluation and resource utilization of seutellaria baicalensis.
出处
《按摩与康复医学》
2013年第7期133-135,共3页
Chinese Manipulation and Rehabilitation Medicine
关键词
黄芩苷
RP-HPLC
黄芩
baicalin
RP-HPLC
scutellaria baicalensits
