摘要
应用微管吸吮系统和激光共聚焦显微镜、标准固体粘弹性模型， 研究内毒素直接作用对于皮细胞弹性模量Ｋ１ 、Ｋ２ 和粘性系数μ的影响。实验结果表明： 随着内毒素浓度的增加（ ０ ．３１２５μｇ／ｍｌ～１０μｇ／ ｍｌ） ， 弹性模量Ｋ１ 、Ｋ２ 均下降（ 其中Ｋ１ 比Ｋ２ 更为显著） ， 粘性系数μ随着内毒素浓度的增加而增加； Ｋ１ 、Ｋ２ 、μ随着内毒素（０．６２５μｇ／ ｍｌ） 作用时间的延长而降低；内毒素直接损伤血管内皮细胞后的共聚焦图像表明： 内皮细胞的微丝、微管发生重排； 细胞核位移、脱核， 内毒素与细胞膜呈全面浸润状态。研究结果从细胞生物力学的角度， 给出内毒素直接损伤血管内皮细胞的证据。
On the basis of standard solid viscoelastic model and using the micropipette aspiration method and the Laser con-focus, the changes of elastic modulus K1、K2 and viscosity coefficient μ of human umbilical vein endothelial cells(HUVEC), after being directly impaired by lipopolysaccharide(LPS)were investigated. The experiment results indicated that elastic modulus K1、K2 were decreased with the increasing of concentration of LPS(0.3125μg/ml～10μg/ml),in which K1 was more obviously than K2. Viscosity coefficient μ was increased with the increasing of concentration of LPS; and K1、K2、μ were decreased with time of being directly impaired. The con-focus imaging of impaired HUVEC by LPS indicated that the cellular skeleton proteins were reorganized, cellular nucleolus were shifting and dropping, and LPS has infiltrated whole into cellular surface. The experiments testify that LPS can directly impair HUVEC from the view point of biomechanics.
出处
《生物物理学报》
CAS
CSCD
北大核心
1999年第4期804-811,共8页
Acta Biophysica Sinica
基金
国家自然科学基金!(No .39830400)
关键词
内皮细胞
内毒素
粘弹性
直接损伤
微管吸吮
HUVEC LPS Viscoelastic module Direct impairment Micropipette aspiration Laser co-focus
