摘要
目的:建立小鼠放射性十二指肠炎模型.方法:♀Balb/c小鼠93只,随机分为对照组(13只)、4.5 Gy组(13只)、6.0 Gy组(13只)、9.0Gy组(13只)、12.0 Gy组(13只)、13.5 Gy组(13只)、15.0 Gy组(15只),60Co射线对小鼠上腹部一次性照射,在照射后第3.5、7天处死解剖取十二指肠组织做病理切片,并取肠系膜淋巴结、脾和外周血血清检测细胞因子白介素-6(interleukin-6,IL-6)、IL-1、肿瘤坏死因子-(tumor necrosis factor-,TNF-.结果:照射后3.5 d,6个照射组促炎性因子IL-6、IL-1、TNF-均较对照组升高;照射后3.5 d,12.0、13.5、15.0 Gy 3个照射组小鼠十二指肠绒毛高度降低,隐窝深度变浅、数目减少,隐窝腔变大,均出现放射性肠炎改变,13.5、15.0 Gy组死亡较多,12.0 Gy组更能模拟临床放射性十二指肠炎,为研究提供模型.结论:60Co射线一次性腹部照射建立小鼠放射性十二指肠炎模型,12 Gy照射剂量更为合适.
AIM： To develop a mouse model of radiation- induced duodenitis. METHODS： Ninety-three female Balb/c mice were randomly divided into seven groups： a con- trol group （n = 13）, a 4.5 Gy group （n = 13）, a 6.0 Gy group （n = 13）, a 9.0 Gy group （n = 13）, a 12.0 Gy group （n = 13）, a 13.5 Gy group （n = 13） and a 15.0 Gy group （n = 15）. Mice were sacrificed 3.5 or 7.0 d after abdominal irradiation with a single dose of Cobalt-60. Duodenal samples were taken for histopathological analysis, and blood, spleen and mesenteric lymph nodes were sampled to detect the levels of inflammatory factors interleukin-6 （IL-6）, IL-1β and tumor necrosis factor-α （TNF-α）. RESULTS： The levels of inflammatory factors IL-6, IL-1β and TNF-α increased in all the irra- diation groups compared to the control group. The length of villi and the number of crypt were significantly reduced in the 12.0, 13.5 and 15.0 Gy groups compared to the control group on day 3.5, which was consistent with the changes in radiation-induced enteritis. The 13.5 and 15.0 Gy groups had a high mortality, and the 12.0 Gy group was better in simulating clinical radioac- tive duodenitis. CONCLUSION： A mouse model of radiation-in- duced duodenitis can be successfully developed by abdominal irradiation with a single dose （12.0 Gy） of 60Co.
出处
《世界华人消化杂志》
CAS
北大核心
2013年第23期2267-2274,共8页
World Chinese Journal of Digestology
