摘要
核心引物对遗传多样性分析、种质资源鉴定、品种纯度和真实性检测、指纹图谱构建等研究具有重要价值。本研究利用均匀分布于烟草24条染色体的278对SSR引物,对20份亲缘关系相对较远的烟草材料进行初步筛选,筛选出32对引物。再加上10份亲缘关系较近的材料,共30份烟草材料对32对引物进行复筛,最终确定14对为核心引物,并通过12份系谱已知的烟草材料进行有效性验证。利用14对核心引物对39份烟草材料进行遗传多样性聚类分析,与农艺性状聚类分析相比较,结果表明该套SSR核心引物适用于烟草种质资源鉴定和遗传多样性分析。
Core primers are important for genetic diversity analysis, germplasm identification,purity and genu- ineness test, and fingerprint establishment. 278 pairs of SSR primers which evenly distributed in 24 chromosomes to- bacco were initial screened on 20 tobacco germplasm stocks with relatively distant,and 32 pairs of primers were se- lected out. Another 10 germplasm stocks with similar relationship were used to screen the 32 pairs of primers and 14 pairs of SSR core primers were obtained. 14 pairs of SSR core primers were conducted on pedigree research,genetic diversity analysis and agronomic traits clustering study in 39 germplasm stocks. The results showed that the core primers could be applied to germplasm identification and genetic diversity study in tobacco.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2013年第5期960-965,共6页
Journal of Plant Genetic Resources
基金
中国烟草总公司重庆市公司"烟草种质资源收集整理及遗传背景分析"项目
