摘要
目的:探讨长期自控锻炼对癌症生存者细胞免疫功能的影响。方法:采用匹配病例对照研究设计,从上海市癌症康复学校学员中选择癌龄10年以上并参加自控锻炼10年以上者30人为锻炼组,另选择年龄、性别、癌症类型、治疗方法等与锻炼组一对一匹配的30人为对照组,对照组不参加规律性锻炼,共计60名。采用癌症病史问卷调查所有受试者既往详细病史,清晨空腹安静状态下肘静脉采血,直接免疫荧光标记全血溶血法测定淋巴细胞表面抗原CD3/CD4/CD8/CD16+56和Tr细胞表面抗原CD4/CD25的表达。结果:锻炼组CD3+T细胞百分比和绝对值均有高于对照组的趋势,CD4+和CD8+与对照组比较无显著性差异;锻炼组CD4+CD25+Tr细胞百分比和绝对值均显著低于对照组;锻炼组CD(16+56)+有高于对照组的趋势;CD4+CD25+Tr与CD(16+56)+、淋巴细胞总数均呈负相关。结论:自控锻炼降低了CD4+CD25+Tr细胞对癌症生存者总体免疫功能的抑制作用,也降低了CD4+CD25+Tr细胞对NK细胞介导的细胞免疫功能的抑制、下调作用。
The purpose of this study was to investigate the effects of self-control exercise on cellular immunity in long-term cancer survivors. Sixty survivors from Shanghai Cancer Rehabilitation Club were recruited for the re- search. Thirty survivors who had taken self-control exercise for more than ten years were included in the exercise group while thirty survivors never practiced self-control exercise in the control group. Age, gender, cancer type and treatment of both groups were carefully matched. A cancer history survey was carried out. Blood was extracted from cubital vein early in the morning. Lymphocyte surface antigen expression such as CD3/CDd/CD8/CD16＋56 and CD4CD25 were determined by direct immunofluorescence staining and flow cytometry. The results showed that per- centage and absolute value of CD3＋T of the exercise group was higher than that of the control group while percentage and absolute value of CD4＋ and CD8＋ was lower. CD （16＋56） of the exercise group tended to be higher than that of the control group. There were no obvious difference in CD4＋ and CDS＋o Tr cell of CD4＋CD25＋ was negatively re- lated to CD （16＋56） ＋ and total number of lymphocytes. It could be concluded that self-control exercise had de- creased the functions of Tr cell of CD4 ＋CD25＋ on general immunity as well as the inhibition of Tr cell of CD4＋ CD25＋ on NK cell＇s immunity.
出处
《武汉体育学院学报》
CSSCI
北大核心
2013年第8期68-72,共5页
Journal of Wuhan Sports University
基金
国家自然科学基金项目(31260251)
上海市重点实验室项目(11DZ2261100)
关键词
自控锻炼
癌症生存
肿瘤免疫
细胞免疫
self-control exercise
cancer survivor
tumor immunity
cellular immunity
