摘要
目的观察脓毒症患者外周血单核细胞CD14^＋CD16-、CD14^＋CD16＋、CD14-CD16^＋三个亚群表达HLA-DR、CD14、CD16的异同，并分析CD14^＋CD16^＋单核细胞的特点。方法选取67例脓毒症患者为脓毒症组和15例健康志愿者作为对照组，脓毒症组根据疾病严重程度分为脓毒症（15例）、重症脓毒症（41例）和脓毒性休克组（11例），收集临床资料，计算APACHEII评分，流式细胞仪检测单核细胞各亚群CD14、CD16、HLA—DR的表达。结果脓毒症患者CD14^＋CD16^＋单核细胞数目较正常对照组显著升高（P〈0．01）；脓毒症患者单核细胞HLA—DR、CD14平均荧光强度均较健康对照组明显降低，并随着脓毒症患者病情由轻到重而逐渐下降；各组中CD14^＋CD16^＋单核细胞相对高表达HLA—DR、CD14；单核细胞HLA—DR表达与临床指标间存在较好的相关关系。结论①脓毒症患者单核细胞HLA—DR表达显著降低；@CD14^＋CD16^＋单核细胞在脓毒症发病的免疫机制中可能起到重要作用。
Objective To observe the expression of HLA-DR, CD14 and CD16 for the different subgroup of monocyte, and analyze the feather of CD14^＋CD16^＋ monocyte. Methods Sixty-seven cases admitted to Emergency ICU and Surgery ICU of the hospital between March and August, 2009 were divided into sepsis group （n=15）, severe sepsis group （n=41）, and septic shock group （n=11）, versus the control group of healthy people （n=15）. The frequencies of CD14, CD16 and HLA-DR of monocyte were detected by flow cytometric analysis. Simultaneously, clinical data were collected and to calculate APACHE II scores. Results A significant increase in the number of CD14^＋CD16^＋ monocyte of sepsis patients were observed compared with the control group; MFI of HLA-DR, CD14 were significantly lower of sepsis group than the control group, and MFI reflected lower with Exacerbations. HLA-DR and CD14 were highly expressed in CD14^＋CD16^＋ monocyte. HLA-DR expression of monocyte is mutually related with the clinical indicators. Conclusion ① HLA-DR Expression of monocyte in sepsis patients reflects significantly lower;② CD14^＋CD16^＋ monocyte might play a key role in the Immune pathogenesis of sepsis.
出处
《中国急救复苏与灾害医学杂志》
2013年第8期717-720,共4页
China Journal of Emergency Resuscitation and Disaster Medicine
