摘要
目的探讨维生素C(Vit C)对MPP+致帕金森病细胞模型中PC12细胞凋亡的影响及作用机制。方法培养神经细胞株PC12细胞,将其分为单纯帕金森病组(MPP+组)和Vit C治疗组(Vit C组),Vit C组加入250μmol/L Vit C孵育6 h,之后向两组均加入100μmol/L MPP+,在体外模拟帕金森病的神经损伤,孵育12 h后,利用乳酸脱氢酶(LDH)试剂盒检测LDH变化;TUNEL法检测鼠脑神经元凋亡情况,并利用Western-blot检测神经元中c-Caspase3和Caspase表达情况。结果与MPP+组比较,VitC能明显减轻帕金森病PC12细胞凋亡,显著改善细胞生存环境;TUNEL检测结果显示,Vit C能明显减少PC12细胞凋亡。同时,Western-blot结果提示Vit C下调c-Caspase3蛋白的表达,组间差异有统计学意义(P<0.05)。结论 Vit C可以直接抑制c-Caspase3表达,减少细胞凋亡,可能对帕金森病造成的细胞凋亡具有一定的拮抗作用。
Objective To investigate the effect and the related mechanism of Vitamin C （Vit C） on PC12 cells apoptosis in MPP＋ induced cell model of Parkinson＇s disease （PD）. Methoda PC12 cells were divided to MPP＋ induced cell model of PD group （MPP＋ group） and Vit C treatment group （Vit C group）. Firstly, Vit C group was incubated with 250 μmol/L Vit C 6 h, and then the two groups were treated with 100 μmol/L MPP＋ 12 h. The LDH concentration was measured by LDH kit. The TUNEL method was performed to measure PCI2 cells apoptosis in two groups. The expression of Caspase3 and c-Caspase3 was detected by Western-blot. Reaults Compared with MPP＋ group, Vit C significantly inhibited LDH concentration and decreased apoptosis of PC12 cells, indicating that Vit C could down-regulate the expression of c-Caspase3 （P〈0.05）. Conclusion Vit C can decrease apoptosis of PC12 ceils in vitro significantlyand play important protection role in PD via down-regulation of the expression of c-Caspase3
出处
《中国临床解剖学杂志》
CSCD
北大核心
2013年第4期453-456,共4页
Chinese Journal of Clinical Anatomy
基金
陕西省教育厅基金(2013JK0755)
陕西省卫生厅基金(2010D57)
西安医学院博士科研启动基金(2012DOC11)
西安医学院校级基金(2012FZ14)
