摘要
目的:探讨CD8+CD28-调节性T细胞在白细胞降低性肺炎发病机制的作用。方法:收集白细胞降低的肺炎患者45例,通过痰及血培养法确定其病原体类型;在连续进行痰培养的3天内采集外周血,采用流式细胞术检测外周血CD8+CD28-调节性T细胞亚群的数量,并且将该细胞的数量与病原体的滴度进行相关性分析;收集36例志愿者作为对照组。结果:与对照组比较,肺炎组患者的CD8+CD28-细胞含量明显升高(P<0.05);从自身变化看,肺炎组治疗第1天的CD8+CD28-含量最多,第2天次之,第3天显著低于前两天(P<0.05)。45例患者的病原体依次为:肺炎支原体阳性20例(44.4%,A组),上呼吸道病毒阳性18例(40.0%,B组),衣原体阳性7例(15.6%,C组);A组CD8+CD28-T细胞显著高于B及C组(P<0.05),而B与C组无统计学差异;相关性分析显示CD8+CD28-与支原体的相关性最显著(P<0.05)。结论:CD8+CD28-T细胞在白细胞降低性肺炎发病机制中起重要作用,其含量有助于支原体肺炎的早期诊断。
Objective：To explore the Relation between CD8＋CD28-regulatory T cells and the pneumonic pathogen with leucopenia for patients with pneumonia.Methods：Collection of white blood cells reduce 45 cases,to determine the pathogene via bacterial culture of sputum and blood,pneumonia in patients sputum culture in continuous 3 days to collect peripheral blood,the flow cytometry test peripheral blood CD8＋CD28-the number of regulatory T cells,and to the number of the cells and pathogens the drop degree of correlation analysis;Volunteers collected 36 cases as control group.Results：Compared with control group,the contents of CD8＋CD28-cells in patients with pneumonia increased significantly（P0.05）;Look from itself changes,day 1 of CD8＋CD28-most abundant,followed the next day,significantly less than two days before the day 3（P0.05） for the patients.For the etiology test of 45 patients with pneumonia,mycoplasma positive（44.4%,group A） and 20 patients with upper respiratory tract virus positive 18 cases（40.0%,group B）,chlamydia positive in 7 cases（15.6%） of group C;A group of CD8＋CD28-T cells is significantly higher than B and C group（P0.05）,while B and C group had no statistical difference;Correlation analysis showed that the correlation of CD8＋CD28-and mycoplasma is the most significant（P0.05）.Conclusion：CD8＋CD28-T cells on the mechanism of white blood cells reduce pneumonia play an important role,its content will help the early diagnosis of mycoplasma pneumonia.
出处
《长治医学院学报》
2013年第4期253-256,共4页
Journal of Changzhi Medical College
