摘要
目的观察疏风宣肺和解表清里方药对流感病毒性肺炎小鼠肺组织CD4＋辅助性T细胞1／2（Thl／2）平衡的调控作用。方法选择SPF级雄性ICR小鼠108只，按随机数字表法分为对照组、模型组、达菲组以及疏风宣肺方高、中、低剂量组和解表清里方高、中、低剂量组9组，每组12只。采用流感病毒亚甲型鼠肺适应株（FMl）滴鼻感染小鼠建立流感病毒性肺炎模型，对照组以0．05ml生理盐水滴鼻。于病毒感染后2h，对照组、模型组灌胃蒸馏水；达菲组给予达菲2．5g·ml-1·d-1；其余6组分别灌服疏风宣肺抗流感方药（主要药物金银花、连翘、牛蒡子、蝉蜕、荆芥等）高、中、低剂量（3．762、1．881、0．941g·kg-1·d-1）和解表清里抗流感方药（主要药物炙麻黄、生石膏、黄芩、杏仁、生甘草等）高、中、低剂量（4．368、2．184、1．092g·kg-1·d-1）；均每日1次，每次0．2ml，连用4d。采用实时荧光定量聚合酶链反应（PCR）和蛋白质免疫印迹法（Westernblotting）分别测定肺组织1-干扰素（IFN-1）、白细胞介素-4（IL-4）mRNA以及蛋白表达水平。结果与对照组比较，模型组IFN-叮mRNA及蛋白表达均明显降低，IL-4mRNA及蛋白表达均明显升高（均P〈0．05）。与模型组比较，各药物组IFN-1mRNA及蛋白表达均升高，以疏风宣肺方低剂量组和解表清里方中剂量组升高更显著（IFN-1mRNA：0．85±0．14、0．58±0．06比0．15±0．48，均P〈0．01）；IL-4mRNA及蛋白表达均有所降低，以疏风宣肺方中剂量组降低更显著（IL-4mRNA：1．06±0．19比2．89±0．75，P〈O．05）。结论疏风宣肺方通过升高IFN-1水平、降低IL-4水平，纠正Thl／2的平衡，减轻肺组织免疫病理损伤，以中低剂量组为优。
Objective To investigate regulatory effects of Shufeng Xuanfei and Jiebiao Qingli Formulae on CD4＋ helper T cell 1/2 （Thl/2） cytokines of mice with influenza virus FM1 pneumonia. Methods One hundred and eight male mice, ICR grade, were randomly divided into nine groups ： control group, model group, oseltamivir group, the high, middle and low dose groups of Shufeng Xuanfei Formula and the high, middle and low dose groups of Jiebiao Qingli Formula （each, n=12）. The pneumonia model was established by dropping influenza virus strain FM1 into the nose, while the control group was given normal saline 0.05 ml into the nose. After the above infectious treatment of mice for 2 hours, they were treated with oseltamivir 2.5 g＂ ml-~＂ d-~ for 4 days in oseltamivir group, high, middle and low doses （3.762,1.881,0.941 g kg-1 d-l ） of Shufeng Xuanfei Formula （the main ingredients including honeysuckle, forsythia, burdock, periostraum cicada, schizonepeta, et al.） and high, middle and low dose （4.368,2.184, 1.092 g-1kg-1 d-1） of Jiebiao Qingli 1Formula[ mainly ephedra, gypsum, scutellaria baicalensis, apricot （almond）, raw glycyrrhiza, et al. ~ were administered intragastrically in the corresponding groups, 0.2 ml once a day for 4 days ; in the control and model groups, the mice were treated by intragastrical administration of the same amount of normal saline for 4 days. The mRNA and protein expressions of interferon-＂/ （IFN- ~ ） and interleukin-4 （IL-4）were determined by real-time fluorescent quantitative polymerase chain reaction （PCR） and Western blotting. Results Compared with control group, in model group, the expressions of IFN- ＂/ mRNA and protein were significantly down-regulated, while those of IL-4 mRNA and protein, obviously up-regulated （all P〈 0.05）. Compared with model group, in all the drug treatment groups, the expressions of IFN-y mRNA and protein were significantly up-regulated, especially prominent in the low dose group of Shufeng Xuanfei Formula and the mid
出处
《中国中西医结合急救杂志》
CAS
北大核心
2013年第1期1-4,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
关键词
中药方剂
疏风宣肺
解表清里
流感病毒性肺炎
辅助性T细胞
小鼠
Chinese medicine formula
Shufeng Xuanfei
Jiebiao Qingli
Influenza viral pneumonia
Helper T cell
Mouse
