摘要
目的:通过建立多囊卵巢综合征(PCOS)高雄血症动物模型,观察金雀异黄素(GEN)对大鼠子宫和卵巢形态学的影响。方法:按照胰岛素(INS)联合HCG造模法建立实验动物模型,分为阴性对照组、模型组、GEN低、中、高剂量组及雌激素阳性对照组,剂量组分别灌胃给予GEN5、10、20mg/kgbw,雌激素组给予己烯雌酚0.5mg/kg,持续15d,观察动情周期10d。试验结束后,取大鼠各脏器称重,计算脏体比值、卵巢和子宫的面积与体积。显微镜下分析子宫和卵巢组织形态学的变化。结果:与模型组相比,中、高剂量组大鼠的子宫/体比升高,差异有显著性(p<0.05);各剂量组大鼠卵巢面积和体积均下降,雌激素组下降明显,有显著性差异(p<0.05)。卵巢大体和镜下观察结果显示,高剂量GEN可使造模后大鼠的卵巢卵泡膜细胞层变薄,颗粒细胞层数明显增多,形态完整,排列整齐,黄体组织数量增多,成熟卵泡居多,囊肿卵泡明显减少。结论:GEN对PCOS高雄血症大鼠的子宫和卵巢形态学起到了改善作用,推测GEN的植物雌激素样作用可能会对PCOS患者发挥治疗作用。
Objective： To establish the polycystic ovary syndrome （PCOS） hyperandrogenism animal models, the effect of genistein（GEN） on morphology of uterine and ovarian was analyzed in this study. Methods： The insulin（INS） combined with HCG molding method was used to establish PCOS animal model. Sixty rats were divided into control group, model group, low dose group, middle dose group, high dose groupand estrogen group, respectively given GEN 5, 10, 20 mg/kg BW, estrogen given diethylstilbestrol 0.5 mg/kg, all compounds were administered intragastric administration on 15 consecutive days and observed estrous cycle 10 days. Effects on wet weight, height of the epithelium and the morphology of uterine and ovarian were examined. The dirty body ratio, area and volume of ovary and uterus were calculated. Results： compared with the model group, there was a significant difference of ovary to body weight ratio in middle and high dose group（p0.05）. The uterine area and volume increased in dose groups, and there was a significant difference in estrogen group（p0.05）. Microscopic observation results showed that high dose GEN could make ovarian theca cell layer to thin in model group rats, numbers of granule cell increased significantly, completely and orderly, luteal tissues increased, mature follicles were majority and cystic follicles reduced significantly. Conclusions： GEN has the improve function in uter- ine and ovarian of PCOS hyperandrogenism rats. It is presumed that the plant estrogenic effects of GEN maybe has a therapeutic action on PCOS patients.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2013年第5期11-16,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金-青年基金项目(81102136)
