摘要
目的 :探讨抑癌基因p53突变和人类乳头状瘤病毒 16,18型 (HPV16,18)感染与喉鳞癌的关系及二者在喉鳞癌中的相关性。方法 :应用聚合酶链反应结合单链构象多态性分析 (PCR -SSCP)技术对4 0例喉鳞癌组织中HPV16,18DNA及p53基因第 5,7外显子的突变进行检测。结果 :HPV16,18的检出率分别为 35% ( 14/ 4 0 )和 10 % ( 4 / 4 0 )。 12例 ( 30 % )标本p53基因突变 ,其中第 5外显子 10例( 2 5% ) ,第 7外显子 2例 ( 5% ) ,p53基因在HPV阳性和阴性标本中的突变率分别为 33 3%和 2 7 3% ,二者差别无显著性 ( χ2 =0 1732 ,P >0 0 5)。结论 :HPV感染和p53基因突变在喉鳞癌的发生发展过程中都起重要作用 。
Objective:To evaluate the role of p53 gene mutations and human papillomavirus type 16,18(HPV16,18) infection in laryngeal squamous cell carcinoma and to investigate the association between HPV infection and p53 gene mutations.Methods:HPV 16,18 DNA and mutations of exons 5 and 7 of p53 gene in 40 laryngeal squamous cell carcinoma tissuses were investigated by polymerase chain reaction(PCR) and PCRsingle stranded conformation polymorphisms analysis.Results:The positive rates of HPV 16 and 18 were 35%(14/40) and 10%(4/40) respectively.12 of 40 cases(30%) revealed p53 gene mutations(10 in exon 5 and 2 in exon 7).The rates of p53 gene mutations in HPVpositive patients and HPVnegative patients were 33.3% and 27.3% respectively. There was no significant difference between them(χ2=0173 2,P>005).Conclusions:HPV infection and p53 gene mutations are two important factors in laryngeal carcinogensis,but there are no correlation between HPV infection and p53 gene mutations.
出处
《肿瘤防治杂志》
2000年第4期360-361,共2页
China Journal of Cancer Prevention and Treatment
关键词
喉肿瘤
人乳头瘤病毒
抑癌基因P53
PCR
laryngeal neoplasms
papillomavirus,human
p53 gene
polymerase chain reaction
singlestranded conformation polymorphisms analysis
