摘要
采用QuEChERS方法结合超高效液相色谱-串联四极杆质谱技术,建立食品中生物碱、真菌毒素、农药等73种有毒有害物质的快速筛查方法。样品经DisQuE提取管提取,DisQuE净化管净化,采用BEH C18液相色谱柱分离,进行梯度洗脱,采用多反应监测(MRM)模式,外标法定量。结果表明:73种有毒有害物质的方法检出限为0.1～50μg/kg;线性范围内低、中、高3个添加水平的回收率为62.05%～112.75%,相对标准偏差(RSD)为3.11%～10.04%。该方法具有操作便捷、通用性强等特点,适用于食品中上述物质的批量快速筛查工作。
An ultra performance liquid chromatography-mass spectrometry（UPLC-MS-MS） with QuEChERS method has been developed for the determination of 73 toxic and harmful substances in foods.Samples were extracted by DisQuE extraction,cleaned up with DisQuE clean-up tube,and chromatographic separation was performed on a BEH C18 column with gradient elution and the analytes were identified by multiple reaction monitoring（MRM） and quantified by external standard method.The results showed that the limits of quantification（LOQs） for 73 toxic and harmful substances were 0.1-50 μg/kg.The recoveries at low,medium and high spiked levels and the relative standard deviations（RSD） were 62.05%-112.75% and 3.11%-10.04%,respectively.This method proved to be rapid,simple,and suitable for the rapid screening of toxic and harmful substances in foods.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第16期214-220,共7页
Food Science
基金
北京市科委计划项目(Z111100074211019)
关键词
QUECHERS
超高效液相色谱串联质谱
生物碱
真菌毒素
农药
筛查
ultra performance liquid chromatography-mass spectrometry（QuEChERS）
ultra performance liquid chromatography-mass spectrometry（UPLC-MS-MS）
alkaloids
mycotoxins
pesticides
screening
