摘要
目的建立高效液相-蒸发光散射检测法（HPCL-ELSD）测定玉屏风胶囊中黄芪甲苷含量的方法。方法色谱柱为phenomenexGenini（5μm,4.6×250mm,C18）;流动相为乙腈-水（75∶25）;流速为1.0mL/min,柱温：30℃。蒸发光散射（ELSD）检测条件：漂移管温度为70℃,雾化管温度为40℃。结果黄芪甲苷在0.3038~1.519μg范围内呈良好的线性关系,r=0.9997,平均加样回收率为98.36%,RSD为0.58%（n=6）。结论本法简便快捷,具有良好的精密性和稳定性,测定结果可靠,可用于本产品的质量控制。
Objective To establish high performance liquid - evaporative light-scattering method （HPCL-ELSD） astragalus armour glycosides content in the jade screen capsule. Methods Chromatographic column for phenomenex Genini（5μm,4.6×250mm,C18）; mobile phase of acetonitrile-water（75 ： 25）; flow velocity is 1.0mL/min; column temperature is 30℃. Evaporative light scattering detection （ELSD） conditions： drift tube temperature is 70℃, spray tube temperature of40℃. Result Astragalus armour glycosides in the 0.3038 g to 0.3038 g scope had good linear relationship r=0.9997, average sample recovery rate was 98.36%, RSD was 0.58%（n=6）. Conclusion This law is simple and quick, with good precision and stability, and the determination results and reliable, and can be used for quality control of this product.
出处
《中国医药指南》
2013年第23期8-9,共2页
Guide of China Medicine
