摘要
目的:结合渗透汽化和流动注射技术,建立一种高锰酸钾-罗丹明B化学发光测定食品中亚硫酸盐的新方法。方法:在酸性介质中,高锰酸钾氧化罗丹明B产生微弱的化学发光,SO2的存在可显著增强该体系的发光信号强度;且在一定范围内呈良好的线性关系;渗透汽化技术在酸性条件下将样品中的亚硫酸盐转化为SO2并分离出来,基于此,利用渗透汽化与化学发光联用技术进行食品中亚硫酸盐含量测定。结果:Na2SO3浓度0.8 mg/L^4.0 mg/L范围内与发光强度呈良好的线性关系,检测限是0.2 mg/L。对2.0mg/L的Na2SO3标准溶液平行测定11次,平行测得值的RSD为4.2%,用于测定食品中亚硫酸盐含量,结果满意。结论:该方法可以用于测定食品中亚硫酸盐含量。
Objective： A chemiluminescence （CL） procedure was developed by pervaporation and flow injection of potassium permanganate - rhodamine B for determination of contaminated sulphite in food samples. Methods： The method is based on the oxidation＇of rhodamine B by permanganate solution in acid medium to give out weak CL signal, which is dramatically enhanced by sulphite which can be converted to sulphur dioxide and transported in per- vaporation module. The CL signal is linear with sulphite concentration in a certain range. Results： Under optimum conditions, the CL emission showed linear with Na2 SO3 in the concentration range of 0.8 mg/L -4.0 mg/L, the de- tection limit was 0.2 mg/L. The relative standard deviation was 4.2% when the eoneentration of Na2SO3 was2.0 mg/L. Conclusion： The method is applied to the determination of sulphite in different food samples with satisfying results.
出处
《中国卫生检验杂志》
CAS
北大核心
2013年第8期1846-1848,1851,共4页
Chinese Journal of Health Laboratory Technology
基金
河北省科技支撑计划项目(11231005D-5)
关键词
亚硫酸盐
渗透汽化
流动注射
化学发光
高锰酸钾
罗丹明B
Sulphite
Pervaporation
Flow injection
Chemiluminescence
Potassium permanganate
Rhoda- mine B
