摘要
试验旨在研究乳房基底部移植骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)对乳腺炎大鼠血清中髓过氧化物酶(plup peroxidase,MPO)、乳过氧化氢酶(milk catalase,LP)及免疫球蛋白M(IgM)、免疫球蛋白A(IgA)、免疫球蛋白G(IgG)的影响。从70只SD正常雌性大鼠中随机选取5只,于产后72h,即试验-1d屠宰,作为正常对照,同时其余65只参照国内常用建立乳头管灌注内毒素的方法,从大鼠乳房基底部注入0.05mL(50μg)内毒素。乳腺炎模型建立成功1d后,即试验0d,随机选5只屠宰作为对照,同时将其余60只随机分为3组,即对照组(乳房基底部注射生理盐水50μL)、抗生素组(乳房基底部注射抗生素50μL(5000U/侧))和移植BMSCs组(乳房基底部移植BMSCs 50μL(1×106个)),每组20只,于试验1、3、5和7d各组分别屠宰5只,所有屠宰大鼠从心脏处采血制备血清,送检血清中MPO、LP、IgM、IgA和IgG含量。结果表明,治疗后各时间点移植BMSCs组血清中MPO含量均显著低于对照组(P<0.05),1和7d时LP含量显著低于对照组(P<0.05)。1和5d时移植BMSCs组IgA、IgG和IgM含量均显著高于对照组(P<0.05),7d时IgG和IgM含量显著高于对照组(P<0.05),1和7d时IgG含量、3和5d时IgA含量及5d时IgM含量均与抗生素组差异显著(P<0.05)。因此,BMSCs和抗生素都能降低乳腺炎大鼠血清中MPO和LP含量,提高IgM、IgA和IgG含量,且移植BMSCs组对抗体的总体作用效果比抗生素组显著。
The aim of the study was to investigate the effects on plup peroxidase （MPO）, milk catalase （LP） and immune globulin-M （IgM）, immune globulin A （IgA）, immune globulin-G （IgG） levels in serum of experimental mastitis that trans- planted rat bone marrow mesenchymal stem cells （BMSCs） through the breast base. 5 rats selected from 70 normal femal SD rats were slaughted after 72 h of postpartum, namely test -1 d, at the same time the rest of 65 rats were perfused 0.05 mL （50 μg） endotoxin through the nipple tube as the reference to domestic common way, 5 rats that the data obtained from these rats would be equally compared and contrasted with data from other groups later were randomly selected and slaughted after 1 d namely test 0 d that established experimental mastitis model successfully, at the same time the rest of the 60 rats were random- ly divided into three groups. The control group （injection of saline in breast base, 50/~L）, antibiotic group （injection of antibi- otic in breast base ,50μL （5000 U/side）） and BMSCs transplantion group （injection of BMSCs in breast base μL （1 ×106 ））, and slaughted 5 rats each group at 1, 3, 5, 7 d respectively, cardiac blood was collected from the dead bodies and preserved for detecting serum MPO, LP, IgM, IgA and IgG. The results showed that compared with control group, the MPO level of BM- SCs transplantion group was significantly lower at any time points after treatment （P〈0.05）, and LP level at 1, 7 d （P〈 0.05）. IgA, IgG and IgM level at 1, 5 d and IgG, IgM level at 7 d were significantly higher than control group （P〈0.05）, IgG level at 1 and 7 d, IgA level at 3 and 5 d, IgM level at 5 d were significantly differences compared with antibiotic group （P〈0.05）. So the level MPO and LP of antibiotic and BMSCs groups were lower, and improved the levels of IgM, IgA and IgG in mastitis serum of rats, and BMSCs transplantion group was more efficient than antibiotic group overall.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第7期112-115,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家奶产业体系(CARS-37)
"十二五"国家科技支撑项目<牧区及农牧交错带奶牛健康养殖技术集成及产业化示范>(2012BAD12B09)
<新疆肉牛安全高效饲养关键技术研究与示范>(2011BAD47B02)
不同用途马高效
安全养殖共性技术研发(2012BAD45B01)
自治区重大专项<新疆农区奶牛健康养殖及疫病防控技术推广与示范>(20101230694)
关键词
骨髓间充质干细胞
乳腺炎
酶
抗体
bone marrow mesenchymal stem cells （BMSCs）
mastitis,enzyme
antibody
