摘要
以长寿花叶片为外植体,对不定芽的诱导及试管苗的增殖进行了研究,结果表明:长寿花叶片一步成苗的适宜培养基配方为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+蔗糖3%+琼脂粉6.5 g/L,试管苗在MS基本培养基上增殖系数较高,试管苗生长健壮,所以MS是试管苗增殖的适宜培养基。
This study took leaf slice of Kalanchod blossfeldiana as explants ,carried on adventitious buds induction and test-tube seedlings propagation ,the results were as follows :the buds induction suitable euhure medium was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+3% suerose+agar 6.5 g/L, proliferation coefficient was higher when plantlets in MS basic medium, so MS was the suitable medium for plantlet proliferation.
出处
《现代农业科技》
2013年第16期145-145,163,共2页
Modern Agricultural Science and Technology
基金
三门峡职业技术学院科研项目(SZY-2012-039)
关键词
长寿花
不定芽
诱导
增殖
Kalanchodblossfeldiana
adventitiousbuds
induction
propagation