摘要
目的 检测白细胞介素 2 (IL— 2 )、肿瘤坏死因子α(TNFα)与顺铂 (DDP)合用对肺腺癌 A5 49细胞株(A5 49)和耐顺铂 A5 49细胞株 (A5 49/DDP)的细胞毒性作用。方法 采用四氮唑蓝快速比色法 (MTT法 )进行体外细胞毒性实验。结果 IL— 2与顺铂使用后顺铂对两株细胞的毒性作用无明显变化 ;TNFα与顺铂用后顺铂对两株细胞的毒性作用明显增强 ;IL— 2、TNFα与顺铂三者合用后顺铂对两株细胞的毒性作用进一步增强。结论 TNFα与顺铂具有协同抑制肺腺癌 A5 49细胞的作用 ,TNFα能增加 A5 49/DDP细胞对顺铂的敏感性 ,IL—
Objective To examine the cytotoxicity of cisplatatin(DDP)combined with Interleukin 2(IL 2) or/and Tumor necrosis factor α (TNF α)against Lung adenocarcinoma A549 cell line(A549)and DDP resistant A549 cell line (A549/DDP).Methods Cytotoxic testing in vitro were performed by MTT assay.Results The cytotoxicity of DDP alone against the two cell line(A549 and A549/DDP)were not apparently different from that of DDP Combined with IL 2,bue weve significantly incleased with the TNFα combination,and enhanced further by adding IL 2.Conclusion The combined use of TNF α and DDP had synergic cytotoxicity against A549.TNFα could increased the sensibility of DDP against A549/DDP. IL-2 could enhanced above two effects further.
出处
《四川医学》
CAS
2000年第6期490-492,共3页
Sichuan Medical Journal
关键词
白细胞介素2
TNFΑ
顺铂
肺肿瘤
药物疗法
Interleukin-2 Tumor necrosis factorα Cisplatatin Lung neoplasm Cell line
