摘要
利用响应面法对海洋细菌Bacillus cereus OPF-0031产普鲁兰酶发酵条件进行优化。在单因素实验基础上,首先通过两水平的PB(Plackett-Burman design)设计筛选出影响普鲁兰酶发酵的四个显著因素:硫酸铵、硫酸镁、硫酸铁、糯米粉;通过4因素4水平的响应面二次回归中心组合实验(central composite design,CCD)对上述影响因素进行优化。实验结果运用Design-Expert软件进行回归分析,最终确定最佳的培养条件:糯米粉15.53 g/L,硫酸铵9 g/L,硫酸镁0.9 g/L,硫酸铁0.018 g/L,蛋白胨10 g/L,磷酸二氢钾0.5 g/L反应温度为30℃,转速为180 r/min,最终酶活可达到2.7742 U/mL。
Response surface methodology （RSM） was applied to optimize the fermentation for producing pullulanase by marine bacterium Bacillus cereus OPF-0031. Based on the result of single factor experiment, four significant factors influ- encing enzyme synthesis were screened by Plackett-Burman design experiment, which were the glutinous rice surface, （ NH4 ） 2SO4, MgSO4, Fe2 （ SO4 ） 3, respectively. The factors were optimized according to the 4 factor 4 levels established by the response surface CCD （central composite design）. Design-Expert software was used to analyze the experimental re- sults and the optimum culture conditions were determined： glutinous rice powder 15.53 g/L, （NH4 ）2SO4 9 g/L, MgSO＋ 0.9 g/L, Fe2 （ SO4 ） 3 0.018 g/L, protein peptone 10 g/L, K2HPO4 0.5 g/L, pH 6.5, reaction temperature 30 ℃ and the rotational speed 180 r/min. Under the optimized conditions, the activity of pullulanase was as high as 2. 7742 U/mL.
出处
《工业微生物》
CAS
CSCD
2013年第4期51-55,共5页
Industrial Microbiology
基金
山东省自然基金项目(编号:ZR2010CM050)
中国海洋大学国家大学生创新性实验计划资助项目
