摘要
采用比较蛋白质组学方法,研究丛枝菌根真菌接种条件下玉米叶片蛋白表达谱的动态变化。以玉米叶片为实验材料,运用双向凝胶电泳技术(2-DE)对丛枝菌根真菌接种后玉米叶片差异表达蛋白进行分离,并通过质谱技术和搜索NCBInr数据库对差异蛋白进行鉴定。结果显示,接种后玉米叶片中有21个蛋白差异表达,其中有8个蛋白被成功鉴定,这些蛋白包括At4g15770、resistance protein candidate RGC2、transducinprotein-like(ISS)、similar to dynamin-like protein、PREG-like protein、ATP synthase CF1 alphasubunit以及2个未知蛋白。该研究有助于更清楚认识菌根改善植物生长的作用机制。
The dynamic change of maize leaf protein expression profiles under AMF inoculation was studied by the method of comparative proteomics.The maize leaf was used as the experimental material.The differentially expressed proteins in the maize leaf were separated by 2-DE and identified by mass spectrometry and searching for NCBInr database after AMF inoculation.The results showed that 21 proteins in the maize leaf differentially expressed.Of them,8 proteins were successfully identified,including At4g15770,resistance protein candidate RGC2,transducin protein-like（ISS）,similar to dynamin-like protein,PREG-like protein,ATP synthase CF1 alpha subunit and two unknown proteins.This study was helpful to further understand the mechanism of AM improving plant growth.
出处
《热带农业科学》
2013年第7期40-44,共5页
Chinese Journal of Tropical Agriculture
基金
国家自然科学基金项目"菌根抑制植物摄入土壤砷的生物屏障作用与机理研究"(No.30571105)
关键词
玉米
叶片
丛枝菌根真菌
蛋白
质谱
Zea mays
leaf
abuscular mycorrhizal fungi （AMF）
Proteins
mass spectrometry
