摘要
目的观察转染反义抑制微小RNA-21（miR-21）对A431细胞株中程序性细胞坏死因子4（PDCD4）和磷酸酶和张力蛋白同源物基因（PTEN）表达及细胞凋亡的影响。方法对A431细胞转染反义miR-21（Anti—miR-21），采用免疫细胞化学法检测转染前后A431细胞株中PDCD4和PTEN的表达，采用原位末端转移酶标记（TUNEL）检测转染前后A431细胞株中细胞凋亡。结果细胞组、转染无关序列组和转染miR-21反义寡核苷酸组PDCD4表达平均吸光度（伪）值分别为62340．03±2157．52、55871．36±7281．27和175302．34±14571．90，差异有统计学意义（F=50．12，P〈0．05）；细胞组、转染无关序列组和转染miR．21反义寡核苷酸组PTEN表达伪值分别为19206．26±655．01、19149．26±1058．29和135572．92±4685．05，差异有统计学意义（F=576．54，P〈0．05）；细胞组、转染无关序列组和转染miR-21反义寡核苷酸组凋亡细胞比例分别为9．90％、12．95％和31．63％，差异有统计学意义（F=201．79，P〈0．05）。结论转染Anti-miR-21后A431细胞株中miR．21表达下降，这种抑制可以上调A431细胞株中PDCD4和PTEN的表达，加速肿瘤细胞凋亡。
Objective To explore the expression of programmed cell death 4 （ PDCD4）, phospha- tase tensin homologue （PTEN） and cell apoptosis in A431 cell line before and after anti-microRNA-21 （anti-miR-21） was transfected. Methods Anti-miR-21 was transfected in A431 cell line, the expressin of miR-21 was detected by reverse transcription-polymerase chain reaction （RT-PCR）, the expression of PDCIM and PTEN was detected by immunocytochemistry; cell apoptosis was detected by TdT-mediated dUTP nick end labeling （TUNEL）. Results The expression of PDCD4 in A431 cell transfected by anti- miR-21 （17 5302. 34 ± 14 571.90） was significantly higher than cell group （62 340. 03 ±2157. 52） and NC group （55 871.35 ± 7281.27） （F = 50. 12, P 〈 0. 05 ）; The expression of PTEN in A431 cell trans- fected by anti-miR-21 （ 135 572. 92 ± 4685.05 ） was significantly higher than cell group （ 19 206. 26 ± 655.01 ） and NC group （ 19 149.26 ± 1058. 29） （ F = 576. 54, P 〈 0. 05 ） ; the ratio of apoptosis cell in A431 cell line transfected by anti-miR-21 （31.63%） was significantly higher than cell group （9. 90% ）and NC group （ 12. 95% ） （ F = 201.79, P 〈 0. 05 ）. Conclusion A431 cell line transfected by anti-miR-21 express low level of miR-21, this transfection can up-regulate the expression of PDCD4, PTEN and the rati- o of apoptosis cell in A431 cell line.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第8期1707-1710,共4页
Chinese Journal of Experimental Surgery
