摘要
目的制备精氨酸-甘氨酸-天冬氨酸(RGD)肽修饰的白藜芦醇(Res)纳米颗粒,观察其对大肠癌细胞株SW620的影响。方法制备RGD肽修饰的PEG-PEI纳米载体结合Res后形成纳米复合物。测定该纳米复合物的粒径、包封率和体外释放特性,并检测其对大肠癌SW620细胞的增殖、细胞周期及凋亡的影响,同时设Res作对照。结果所制备的Res纳米复合物平均粒径为(124.9±36.0)nm,包封率为(85.3±5.6)%,3d内共释放(72.5±8.3)%的Res。60、80、100μmol/L的Res纳米复合物对SW620细胞的增殖抑制作用与同浓度Res相比显著增强(P<0.05)。40、80μmol/L的Res纳米复合物使SW620细胞的S期细胞比例显著增加,40μmol/L的Res纳米复合物使凋亡细胞比例显著增加。结论 Res纳米复合物对大肠癌细胞株SW620有较好的增殖抑制活性,诱导细胞凋亡,增加细胞进入S期的比例,有着良好的应用前景。
Objective To observe the effects of resveratrol-loaded arginine-glycine-aspartic acid （RGD） peptide modified PEG-PEI nanopartieles on proliferation and cell cycle of eoloreetal cancer cell line SW620. Methods Resveratrol-loaded RGD peptide modified PEG-PEI nanoparticles were constructed. The diameter, encapsulation efficiency and the release rate of the nanoeomposite in vitro and its effeets on proliferation, cell cycle and apoptosis of human colorectal cancer cell line SW620 were measured. Results Resveratrol-loaded RGD peptide modified PEG-PEI nanoparticles were constructed successfully. The average diameter of nanoparticles was （ 124. 9±36. 0） nm, the average encapsulation efficiency was （ 85.3 ± 5.6 ） % , and （ 72. 5 ±8.3 ） % of the resveratrol was released in 3 days. The SW620 proliferation could be significantly inhibited by resveratrol-loaded nanoparticles compared with free resveratrol at the concentrations of 60, 80, and 100p, mol/L（P 〈0. 05）. SW620 in S phase increased significantly by resveratrol-loaded nanoparti- cles at the concentrations of 40 and 80μmol/L, while the proportion of apoptotic cells increased significantly at the concentration of 40μmol/L. Conclusion The resveratrol-loaded nanoparticles are able to induce apoptosis, have antiproliferative activity on human colorectal cancer cell line SW620 and increase the ratio of phase S ceils, which makes a good prospect.
出处
《临床肿瘤学杂志》
CAS
2013年第7期599-602,共4页
Chinese Clinical Oncology
