摘要
目的半定量分析真核细胞翻译起始因子4E(eIF4E)在鼻咽癌组织中的表达及其与各临床病理参数的相关性。方法采用SP免疫组化法检测40例鼻咽癌组织和40例癌旁鼻咽黏膜慢性炎组织中eIF4E蛋白的表达情况,使用Imagepro-plus 6.0软件分析eIF4E蛋白表达的阳性程度。结果鼻咽癌组织及鼻咽黏膜慢性炎组织中eIF4E蛋白表达平均光密度值(MOD值)分别为(0.153±0.041)和(0.109±0.037),二者差异具有统计学意义(P<0.05)。鼻咽癌组织临床分期越晚,阳性表达率越高;分化程度越高,阳性表达率越低;eIF4E表达在淋巴结转移组织中明显高于无淋巴结转移组织(P<0.05)。结论 eIF4E与鼻咽癌的进展及生物学行为有密切关系,可作为判断鼻咽癌恶性程度的指标。
Objective To explore the express＇ion and clinical significance of elF4E protein in nasopharyn- geal carcinoma and its relationship with clinicopathologic features. Methods In 40 patients of nasopharyngeal carci- noma and 40 patients of nasopharyngeal chronic inflammation tissues, the elF4E protein levels detected with SP immu- nohistochemistry method were analyzed semi-quantitatively by Image pro-plus 6.0 image software. Results The mean optical densities （MODs） of elF4E protein in nasopharyngeal carcinoma and chronic inflammation tissues were （0.153±0.041） and （0.089±0.037）, respectively, which shows that the positive expression rate of elF4E in nasopharyn- geal carcinoma were higher than that in chronic inflammation tissues （P〈0.05）. The positive expression of eIF4E was associated with TNM stage and lymphnode metastasis. Conclusion elF4E may play a role in the carcinogenesis and progression ofnasopharyngeal carcinoma.
出处
《海南医学》
CAS
2013年第15期2185-2187,共3页
Hainan Medical Journal
基金
湛江市科技攻关计划项目(编号:2011C3109013)
广东医学院青年基金(编号:Q2011008)
关键词
鼻咽癌
EIF4E
免疫组化
Nasopharyngeal carcinoma
elF4E
Immunohistochemistry
