摘要
以FAM和BHQ1两种荧光素分别标记为报告染料和淬灭染料,建立了转基因发酵豆制品中抗草甘膦转基因EPSPS、大豆凝集素Lectin基因的Taqman荧光PCR定量检测体系,检测灵敏度为0.1%,重现性良好,特异性强,可作为发酵豆制品转基因成分定量检测的供选方法。在霉千张和臭腐乳(青方乳)等发酵豆制品中检出抗草甘膦转基因成分。
With FAM and BHQ1 two fluorescent labeled reporter dye and a quencher dye, the detection system was established about EPSPS （5-enolpyruvylshimate-3-phosphate synthase gen） and Lectin （soybean keeping gene） in fermented soy products by Taqman fluorescent quantitative PCR, the sensitivity was 0.1%, and reproducibility is good. Because of its good repeatability, specificity and reliability, TaqMan probe method is extremely adequate for the quantitative detection of genetically modified component in fermented soy products. The genetically modified EPSPS were firstly detected in mildew Qian Zhang and smelly bean curd fermented soy products.
出处
《食品研究与开发》
CAS
北大核心
2013年第13期79-83,共5页
Food Research and Development
基金
国家质检总局科技项目(2010QK321)
