摘要
利用PCR方法克隆瑞氏木霉(Trichoderma reesei)外切葡聚糖纤维二糖水解酶Ⅰ基因(cbhⅠ)启动子序列,以pSK载体为骨架,构建了pSK-cbhⅠ-GFP-hph表达载体,并成功转入到绿色木霉(T.viride)Sn-9106中。通过对绿色木霉Sn-9106进行遗传改造,为纤维素酶基因在木霉中同源重组表达提供遗传转化平台。
Promoter of cellobiohydrolase I gene （cbh I ） of Trichoderma reesei was cloned by PCR. The expression vector pSK-cbh I-GFP-hph was constructed with pSK vector as backbone. Then it was introduced into the strain of T. viride Sn-9106 by transformation. The genetic modification of T. viride Sn-9106 could provide genetic transformation platform for homologous recombination expression of cellulase gene in T. spp..
出处
《湖北农业科学》
北大核心
2013年第13期3180-3183,共4页
Hubei Agricultural Sciences
