摘要
目的探讨细胞周期素D1（CyclinD1）、血管内皮生长因子（VEGF）和转移抑制因子23（nm23）蛋白在胆管癌（ChC）中的表达及临床意义。方法应用免疫组化方法检测50例ChC和30例癌旁胆管组织（TAC）和20例正常胆管组织（NBDT）中Cyclin D1、VEGF及nm23蛋白表达水平，并结合临床病理特征进行分析。结果在ChC中CyelinD1、VEGF和nrn23蛋白阳性表达率分别为68．0％（36／50）、82．0％（41／50）和54．0％（27／50）。ChC中Cyelin D1和VEGF表达阳性率显著高于TAC和NBDT（P〈0．05），而nm23表达阳性率显著低于TAC和NBDT（P〈0．05）。Cyclin D1、VEGF高表达和nm23低表达与ChC的分化程度、TNM分期和转移相关（P〈0．05）。结论检测Cyelin D1、VEGF和nrn23基因蛋白表达可作为评估ChC生物学行为和预后的参考指标。
Objective To investigate the expression and clinical significance of Cyclin D1, vas- cular endothelial growth factor （VEGF） and non-metastasis 23 （nm23） protein in cholangio- carcinomas （ChC）. Methods Immunohistochemical method was used to test the expression of Cyclin D1, VEGF and nm23 protein in 50 cases of ChC, 30 cases of tissues adjacent to cholangiocarcinoma（TAC） and 20 cases of normal bile duct tissues（ NBDT）, and the clinicopathological factors were also analyzed. Results The positive rate of Cyclin D1, VEGF and nm23 in ChC was 68.0% （36/50）, 82.0% （41/50） and 54. 0% （27/50）, respectively. The expression of Cyclin D1 ,VEGF in ChC was higher than that in TAC and NBDT （P 〈 0. 05 ）. The positive rate of nm23 in ChC was lower than that in TAC and NBDT （ P 〈 0.05 ）. The high level expression of Cyclin D1, VEGF and the low expression of nm23 were positively correlated with the TNM stage, differentiation degree and metastasis in ChC （P 〈 0. 05 ）. Conclusions Expression of tumor suppressor Cyclin D1, VEGF and nm23 gene protein can be reference index to assess the biological behavior and prognosis of ChC.
出处
《中国实用医刊》
2013年第15期60-63,共4页
Chinese Journal of Practical Medicine
