摘要
目的:研究灵孢多糖对α-鹅膏毒肽中毒小鼠肝肾的影响并探索其对α-鹅膏毒肽中毒小鼠肝肾的影响是否存在量效关系。方法:将昆明雄性小鼠48只随机分为六组,即空白组、毒模组、灵孢多糖治疗1～4组,每组8只,复制α-鹅膏毒肽中毒模型4h后,空白组、毒模组腹腔注射生理盐水0.5mL,4个治疗组分别腹腔注射灵孢多糖0.5、1.0、2.0、4.0mL/kg,每天1次,连续3d。观察动物的行为表现及生存状况,实验72h小鼠摘眼球取血后对其进行解剖,计算肝肾脏器系数;检测ALT、AST、BUN、SCR;光镜下观察肝肾的病理组织改变。结果:与毒模组比较,灵孢多糖治疗组小鼠行为表现较好,肝肾脏器系数降低(P<0.05),ALT、AST、BUN、SCR较毒模组下降明显(P<0.05),肝肾病理损害程度减轻。结论:灵孢多糖对α-鹅膏毒肽中毒小鼠的肝肾具有保护作用,在本实验中灵孢多糖对α-鹅膏毒肽中毒小鼠的肝肾保护存在量效关系。
Objective：To exam the effects of Polysacharidum of G.Lucidum Karst on the liver and kidney of α-amanitin-poisoning mice and the existence of dose-effect relationship of these effects.Methods：48 Kunming male mice were randomly divided into 6 groups （8 in each）：blank control group,poisoned model group,Polysacharidum of G.Lucidum Karst treatment groups 1～4.4 hours after the mice models were made,blank control group and poisoned model group were intraperitoneally injected with 0.5 mL normal saline,the mice of treatment groups 1～4 were respectively intraperitoneally injected with 0.5、1.0、2.0、4.0 mL/kg Polysacharidum of G.Lucidum Karst （once a day and for 3 days）.The behavior and survival status of animals were observed.The blood was collected by picking the eyeballs of the mice after 72 hours,and then dissected the animals.Calculated organ coefficient,d etected the data of ALT、 AST、BUN、SCR and observed pathological changes with light microscope.Results：Compared with the poisoned model group,mice in Polysacharidum of G.Lucidum Karst treatment groups were in better behavior,the hepati c and renal organ coefficients lowered down （P0.05）,ALT、AST、BUN、SCR remarkably decreased（P0.05）and the injury was slight in the treatment group.Conclusion：The Polysacharidum of G.Lucidum Karst not only have protective effects on hepatic and nephritic injury induced by α-amanitin in mice,but also have dose-effect relationship with these effects.
出处
《实用中西医结合临床》
2013年第4期1-2,9,共3页
Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
基金
湖南省自然科学基金资助(NO:05JJ30175)
关键词
α-鹅膏毒肽
灵孢多糖
脏器系数
肝肾保护
量效关系
α-amanitin
Polysacharidum of G.Lucidum Karst
Organ coefficient
Hepatic and renal protection
Dose-effect relationship
