摘要
目的探讨绿茶多酚(Green tea polyphenols,GTPs)对癫痫大鼠海马中XIAP、caspase-3表达的影响。方法将雄性Wistar大鼠随机分为正常对照组(NS组)、癫痫组(PTZ组)和绿茶多酚组(GTPs组)。依据Racine分级标准观察各组动物行为学表现,用HE染色观察大鼠海马神经元损伤情况,Western Blot方法测定各组大鼠海马组织中XIAP及caspase-3蛋白表达变化。结果 PTZ组:大鼠海马XIAP表达水平(0.23±0.02)较NS组(0.47±0.05)明显降低(P<0.05);caspase-3蛋白(0.63±0.12)较NS组(0.24±0.07)明显升高(P<0.05)。与PTZ组比较,GTPs组大鼠海马XIAP表达(0.53±0.10)显著升高(P<0.05);caspase-3表达(0.30±0.09)显著降低(P<0.05)。结论癫痫可导致海马组织中抗凋亡蛋白XIAP表达下降,凋亡执行蛋白caspase-3表达增加;而GTPs则能够上调XIAP表达,降低caspase-3的表达,推测GTPs可能通过抗凋亡途径来发挥癫痫后的神经保护作用。
Objective To explore the effect of Green tea polyphenols on the expression of XIAP and caspase-3 in Hippocampus of epileptic rats.Methods Male Wistar rats were randomly divided into three groups,normal control group（NS group）,epilepsy group（PTZ group）,and green tea polyphenols group（GTPs group）.Seizure activity was observed for score according to Racine.HE staining was performed to observe the neuron damage in hippocampus.The expression of XIAP and caspase-3 protein in hippocampus was determined by Western Blot.Results The expression of XIAP protein in PTZ group was decreased obviously compared to NS group（P 0.05）.The expression of caspase-3 protein in PTZ group was increased obviously compared to NS group（P 0.05）.Compared to PTZ group,the protein level of XIAP in the GTPs group was increased significantly;The protein levels of caspase-3 were decreased significantly（P 0.05）.Conclusion Epilepsy could make anti-apoptotic protein XIAP reduced and apoptosis-executed protein caspase-3 increased.Green tea polyphenols could increase the level of XIAP protein and decrease the level of caspase-3 protein.Thus,we speculate that GTPs probably play a neuroprotective role in epilepsy by inhibiting apoptosis.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2013年第7期602-604,共3页
Journal of Apoplexy and Nervous Diseases
基金
河北省自然科学基金资助项目(C2010000548)
关键词
绿茶多酚
癫痫
戊四氮
凋亡
X连锁凋亡抑制蛋白
Green tea polyphenols
Epilepsy
Pentylenetetrazole
Apoptosis
X-Linked Inhibitor of Apoptosis Protein
