摘要
采用鳞茎盘、花蕾、试管苗叶片和假茎为外植体材料,以MS为基本培养基,建立分蘖洋葱再生体系。结果表明,MS+0.5 mg·L-1KT+2.0 mg·L-12,4-D最适合愈伤组织诱导,4种外植体材料中鳞茎盘外植体诱导出的愈伤组织状态最优,出愈率最高达86.4%;愈伤分化丛生芽的最优培养基为MS+0.2 mg·L-16-BA+0.1 mg·L-1NAA,分化率为27.58%;在MS+1.5 mg·L-1IBA+0.01 mg·L-1NAA培养基上可诱导出白色健壮根,生根率可达100%。
This experiment established the tillered-onion regeneration system using the basal bulb, bud, leaves and pseudo stem of test tube seedlings as the explants, with MS as the basic culture medium. The results showed that MS＋0.5 mg. L1 KT＋2.0 mg. L1 2, 4-D was the most suitable for callus induction. Among the four explants, the basal bulball induced the optimum condition callus. The rate of callus induction reached as high as 86.4%. The best medium for multiple buds of callus differentiation was as follows, MS＋0.2 mg . L^-1 6-BA＋0.1 mg . L^-1 NAA, differentiation rate was up to 27.58%. The medium which contained MS＋1.5 mg. L^-1 IBA＋0.01 mg. L^-1 NAA induced white strong roots, rooting rate reached 100%.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2013年第7期96-100,F0003,共6页
Journal of Northeast Agricultural University
基金
公益性行业(农业)科研专项(200903018)
黑龙江省高校重点实验室开放基金项目(GS2009004)
关键词
分蘖洋葱
鳞茎盘
再生
优化
tillered-onion
basal bulb
regeneration
optimization
