摘要
目的 探讨扶正化瘀方对大鼠肝大部切除（PH）后肝再生的影响.方法 将28只正常雄性Wistar大鼠随机分为4组,分别为假手术组（对照组）、PH模型（PH）组、PH＋扶正化瘀组和PH＋促肝细胞生长素（pHGF）组,每组7只.扶正化瘀组和PH ＋pHGF组于实验前3天至实验结束,分别给予扶正化瘀方药液灌胃1 ml/l00 g和pHGF腹腔注射1 ml/100 g,每日1次.术后24 h心脏采血后处死大鼠,计算各组肝再生速度,血清标本检测ALT、AST、肿瘤坏死因子α（TNF-α）和白细胞介素6（IL-6）水平,免疫组织化学染色和荧光定量PCR检测肝组织cyclinD1的表达.结果 PH组、PH ＋pHGF组和PH＋扶正化瘀组ALT、AST水平明显升高,白蛋白水平明显下降,与对照组比较差异均有统计学意义（均P ＜0.01）.与对照组比较,PH组、PH ＋pHGF组和PH＋扶正化瘀组血清TNF-α和IL-6水平均明显升高（均P＜0.01）.与对照组比较,PH组、PH＋扶正化瘀组和PH ＋pHGF组大鼠有丝分裂指数、肝组织cyclin D1 mRNA和肝细胞核cyclin D1阳性率均明显升高（均P＜0.01）；PH＋ PHGF组和PH＋扶正化瘀组肝再生速度明显高于PH组[（22±4）％、（20±2）％比（13±1）％,P＜0.01].与对照组比较,PH组、PH ＋pHGF组和PH＋扶正化瘀组有丝分裂指数、肝组织cyclinD1 mRNA和肝细胞核cyclin D1阳性率均明显升高[有丝分裂指数：（25.90±0.95）,（29.70±0.50）,（30.30±0.96）％比（0.95±0.19）％；肝组织cyclin D1 mRNA：2.18±0.37,2.73±0.29,2.93±0.18比1.03±0.09；肝细胞核cyclin D1阳性率：（27.20±0.36）％,（28.30±0.16）％,（29.30±0.21）％比（0.95±0.19）％]（P＜0.01或P＜0.05）,而PH＋扶正化瘀组和PH ＋pHGF组间差异无统计学意义（P＞0.05）.结论 扶正化瘀方可通过增强cyclinD1表达而促进大鼠肝大部切除术后的肝再生.
Objective To investigate the effect and possible mechanism of Fuzheng huayu decoction on liver regeneration in rats subsequent to partial hepatectomy.Methods Male Wistar rats were randomized into 4 groups：sham-operation rats （control group）,partial hepatectomy rats （PH group）,partial hepatectomy plus Fuzheng huayu decoction rats （PH ＋ Fuzheng group）,partial hepatectomy plus pHGF rats （PH ＋ pHGF group）.24 h after hepatectomy,rats were sacrificed; blood and organs were removed.Serum aspartate aminotransferase,alanine aminotransferase,tumor necrosis factor α （TNF）-α and intedeukin-6 （IL-6） levels were determined.The liver regeneration rate of the rats and the mitotic index were calculated.The gene and protein expression of cyclinD1 in liver tissue were evaluated by real-time PCR and immunohistochemical staining.Results Alanine aminotransferase （ALT） and aspartate aminotransferase （AST） levels were significantly increased and albumin levels were significantly decreased in PH group,PH ＋ pHGF group and PH ＋ Fuzheng group compared with the control group; the differences were statistically significant （P 〈 0.01）.Compared with the control group,serum TNF-α and IL-6 levels in the PH group,PH ＋ the of pHGF group and PH ＋ Fuzheng group were significantly elevated （both P 〈 0.01）.Compared with control group,the mitotic index,the gene and protein expression of cyclinD1 increased significantly in PH group,PH ＋ Fuzheng group and PH ＋ pHGF group at the 24 hour after partial hepatectomy.The mitotic index,cyclinD1 and liver regeneration rate of the rats increased significantly in PH ＋ Fuzheng group and PH ＋ pHGF group than that in PH group.Conclusion Fuzheng huayu decoction can improve liver regeneration by a mechanism related to the up-regulation of cyclinD1 gene expression.
出处
《中国医药》
2013年第8期1121-1123,共3页
China Medicine
基金
河北省二0一一年医学科学研究重点课题计划任（20110112）
关键词
肝再生
肝大部切除
扶正化瘀
Liver regeneration
Hepatectomy
Fuzheng huayu decoction
