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大鼠肝大部切除后肝再生过程中肝小叶结构的重建

Reconstruction of hepatic lobule during liver regeneration after partial hepatectomy in normal rats
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摘要 目的:观察正常大鼠肝大部切除后肝再生过程中肝小叶大小的动态变化,有助于探明大鼠肝再生过程中肝小叶结构的重建过程,并提供组织学依据。方法:正常雄性Wistar大鼠随机分为正常对照组、假手术组和部分肝切除组。采用Higgins和Anderson方法行大鼠70%肝切除,术中即刻称取切除肝叶的湿重。分别于术后12h、24h、72h、120h、1周、2周处死大鼠,留取全部肝组织称湿重,统一留取肝右叶肝组织中性甲醛固定,石蜡包埋连续切片进行H-E染色,显微镜下观察再生肝肝小叶大小变化。结果:正常大鼠部分肝切除后,12h可观察到肝小叶面积开始增大,120h达高峰,从l周开始可见汇管区门静脉终末支的增大并与邻近中央静脉相联系,肝小叶结构逐步重建,2周时肝小叶面积接近正常,小叶数目增加。正常对照组和假手术组大鼠未见上述动态变化。结论:正常大鼠部分肝切除后,肝再生通过早期肝小叶面积增大,后期肝小叶数目增多而恢复。 Objective:To observe the size of the hepatic lobule during liver regeneration after partial hepatectomy (PH) in normal rats. It is helpful to know the hepatic lobular reconstruction during liver regeneration. Methods: The normal male Wistar rats were derided into three groups:normal control group, sham-operated group and PH group. Partial hepatectomy was carried out according to the method of Higgins and Anderson. Liver tissues were obtained at 12 h, 24 h, 72 h, 120 h, 1 week and 2 weeks after PH. One part of the liver was fixed in formalin, then embedded in paraffin, and serial sections were HE stained, to observe the histological changes. Results: The size of the hepatic lobule enlarged at 12 h after PH, and to its maximum at 120 h after PH. The number of the hepatic lobule increased at 1 week after PH, and the liver structure restored normal at 2 weeks after PH. Conclusion: Liver regeneration is a process of dynamic changes from the enlargement of the he parle lobule to the increased number of hepatic lobules till normal structure restores.
出处 《解剖学杂志》 CAS CSCD 北大核心 2012年第4期438-440,共3页 Chinese Journal of Anatomy
基金 河北省卫生厅科研基金(20110112)
关键词 肝再生 肝切除术 肝小叶 重建 liver regeneration hepatectomy hepatic lobule reconstruction
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参考文献8

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