摘要
目的观察阿托伐他汀对Aβ1-40诱导AD大鼠模型的氧化应激和凋亡的影响。方法雄性SD大鼠40只,体质量250～300g,随机分为空白对照组、假手术组、Aβ组、Aβ+生理盐水组、Aβ+阿托伐他汀组,每组8只。后3组在大鼠双侧海马CA1区立体定向注射Aβ造AD模型,假手术组注射生理盐水,正常对照组不做任何处理。Aβ+阿托伐他汀组在造模1周前开始给予阿托伐他汀20mg/(kg.d)灌胃,Aβ+生理盐水组给予等量生理盐水灌胃。造模4周后,测定大鼠脑皮质、海马组织MDA、SOD及GSH活性的变化;蛋白印迹技术检测大鼠海马区Bcl-2及Caspase-3的表达。结果与空白对照组相比,Aβ组大鼠脑皮质、海马组织内MDA活性明显升高(P<0.05),SOD、GSH活性明显降低(P<0.05),Caspase-3的表达升高(P<0.05),Bcl-2的表达降低(P<0.05),与Aβ组相比,Aβ+阿托伐他汀组大鼠的大脑皮质、海马组织内MDA明显减低(P<0.05),SOD、GSH活性明显升高(P<0.05),Caspase-3的表达下降(P<0.05),Bcl-2的表达升高(P<0.05)。结论阿托伐他汀对Aβ1-40诱导AD大鼠模型有一定的脑保护作用,其机制与抑制氧化应激及抗凋亡有关。
Objective To investigate the effects of atorvastatin on anti-oxidative stress and apoptosis in Alzheimer＇s disease rat model induced by Aβ1-40.Methods Fourty healthy Wistar rats were randomly divided into 5 groups,namely,Aβ group,Aβ＋atorvastatin group,Aβ＋NS group,sham-operation group and nomal control group.The first 3 groups were given Aβ to induce AD model and sham-operation group was given normal saline,and then Aβ＋ atorvastatin group and Aβ＋NS group were given corresponding drugs,respcetively.After 4 weeks,the activity of MDA,SOD and GSH in the cortex and hippocampus were measured.And at the same time,the expression levels of Bcl-2 and Caspase-3 were detected by Western blot.Results In the cortex and hippocampus of the model group,the MDA level wac increased（P0.05）,the SOD and GSH levels were decreased（P0.05）.The expression level of Caspase-3 was increased（P0.05）,and the expression level of Bcl-2 was decreased compared with the control group.In the cortex and hippocampus of Aβ＋atorvastatin group,the levels of MDA and caspase-3 were decreased,and the levels of SOD,GSH and Bcl-2 weve significautlg increased.Conclusion Atorvastatin has neuroprotective effects on AD by anti-oxidative stress and anti-apoptosis
出处
《中国实用神经疾病杂志》
2013年第13期6-8,共3页
Chinese Journal of Practical Nervous Diseases
