摘要
利用MSAP(甲基化敏感扩增多态性)技术对11株叶籽银杏的胞嘧啶甲基化水平和模式进行评估。结果表明,叶籽银杏基因组的CCGG序列中检测到36.86%～46.43%的DNA发生甲基化。本研究从36对选扩增引物中筛选出14对引物组合,共得到2879条清晰可辨的带,叶籽银杏总体平均DNA基甲基化水平为42.14%,甲基化模式以内侧胞嘧啶为主,其中,外侧胞嘧啶甲基化水平为11.90%～23.90%,内侧胞嘧啶甲基化水平为17.03%～28.37%。UPGMA聚类分析和主成分分析结果一致,不同单株的叶籽银杏可分为3大类。推断叶籽银杏的变异机制可能与DNA甲基化有关。
The level and pattern of cytosine methylation in 11 Ginkgo biloba L. var. epiphylla Mak.. Individual’s genomes were assessed by using the technique of methylation-sensitive amplified polymorphism （MSAP）. The results showed that 36.86%～46.43% DNA methylation levels were detected in the CCGG sequence of Ginkgo biloba L. var. epiphylla Mak. genomes. Total 2 879 distinguishable bands were amplified by using 36 pairs of selective primers, and 14 of the 36 primers showed polymorphism. The average proportion of DNA methylation was 42.14%. The inner-methylation of double-stranded DNA was the main pattern of the genomic DNA methylation on Ginkgo biloba L. var. epiphylla Mak.. Among these, the outer-methylation and inner-methylation level were 11.90%～23.90% and 17.03%～28.37%, respectively. Cluster analysis using UPGMA was consistent with principal components analysis, the results demonstrated the different individuals could be clustered into three groups.
出处
《分子植物育种》
CAS
CSCD
北大核心
2013年第4期551-558,共8页
Molecular Plant Breeding
基金
国家自然基金项目(32070589)资助
关键词
叶籽银杏
DNA甲基化
MSAP分析
表观遗传学
Ginkgo biloba L. var. epiphylla Mak.
DNA methylation
MSAP analysis
Epigenetics
