摘要
DNA甲基化是一种与细胞和生物体适应紧密关联的表观遗传修饰.本文以松嫩草原多年生草本植物芦苇(Phragmites austrilis)为研究对象,探讨了DNA甲基化在松嫩平原4种天然种群(标记为DBS、QG、SS1和SS2)内和种群间的多态性.采用甲基敏感扩增多态性(MSAP)方法,在4个芦苇种群中发现3个种群的DNA甲基化多态性水平较高,而SS1和SS2种群内个体间甲基化变异相对较高.鉴于SS种群在高pH土壤中生长,而其他2个种群来自低pH土壤,至少部分DNA甲基化变异可能是由盐碱化土壤条件诱导产生的.此外,发现MSAP检测到的甲基化多态性与AFLP和S-SAP检测到的遗传变异密切相关.特异性甲基化多态性条带的同源性分析表明,与已知功能基因具有同源性的基因主要参与细胞代谢和应激反应.
DNA methylation is an evolutionarily conserved epigenetic marker with important relevance to cellular and organismal adaptation.Here,we investigated polymorphism of this marker within and among four natural populations of the perennial grass species Phragmites austrilis,a wetland-grown plant widely distributed in the Songnen Prairie in northeast China.We used the methylation-sensitive amplified polymorphism(MSAP)with a modified scoring criterion and uncovered high levels of DNA methylation polymorphism in three of the four populations of Phragmites austrilis,while the population SS1and SS2 showed relatively high inter-individual methylation variation.Given that the SS population is growing in high pH value soil,whereas the other two populations are from lower pH soil,our results suggest that at least some of the DNA methylation variations are likely resulting from the salinity/alkaline edaphic condition.Another notable observation is that there is close correlations between the methylation polymorphism detected by MSAP and genetic variations detected by amplified fragment length polymorphism(AFLP)and sequence-specific amplification polymorphism(S-SAP)in this set of Phragmites austrilis plants.Homology analysis of a set of population-specific methylation polymorphic bands indicated that of those with homology to known-function genes,they are mainly involved in cellular metabolism and stress-response.
作者
于英杰
石奔
胡兰娟
YU Ying-jie;SHI Ben;HU Lan-juan(College of Life Sciences,Jilin Normal University,Siping 136000,China;Key Laboratory of Molecular Epigenetics of MOE,Northeast Normal University,Changchun 130024,China;Jilin Province Engineering Laboratory of Plant Genetic Improvement,Jilin University,Changchun 130062,China)
出处
《吉林师范大学学报（自然科学版）》
2023年第3期108-117,共10页
Journal of Jilin Normal University:Natural Science Edition
基金
国家自然科学基金项目(32101448)。
关键词
表观遗传变异
适应
天然种群
盐碱胁迫
芦苇
epigenetic variation
adaptation
natural population
salinity-alkaline stress
Phragmites austrilis
