摘要
目的:观察附子理中汤对腹泻型肠易激(D-IBS)大鼠模型结肠组织中toll样受体4(TLR4)表达水平的影响。方法:健康SPF级大鼠50只随机分为正常组、模型组、匹维溴铵组、附子理中汤高、低剂量组,用束缚应激加大黄ig方法联合复制D-IBS大鼠模型,造模成功后附子理中汤高、低剂量组(30,15 g.kg-1)、匹维溴铵(30 mg.kg-1)ig给药,1次/d,连续14 d。采用荧光定量PCR方法观察该模型大鼠结肠黏膜TLR4 mRNA的表达情况。结果:模型组大鼠肠黏膜的TLR4的表达(1.229±0.172)高于正常对照组(1.109±0.062)(P<0.01)。而附子理中汤的干预可使大鼠肠黏膜的TLR4的表达趋于正常,与正常组无明显差异。附子理中汤高(1.068±0.056),低(1.067±0.071)剂量组之间没有显著差异,匹维溴铵组(1.205±0.290)与正常组相比TLR4的表达增多(P<0.01)。结论:附子理中汤可能通过增强整体机体耐受性,降低TLR4的表达,减少免疫应答,来维持肠道的稳态。
Objective： To observe the toll like receptor（TLR） 4 mRNA expression decoction on rat diarrhea type irritable bowel syndrome（D-IBS） colon tissue model by Fuzi Lizhong（FZLZ） decoction.Method： SPF 50 rats were randomly divided into normal group,model group,FZLZ high and low dose group（30,15 g.kg-1）,pinaverium bromide group（30 mg.kg-1）.IBS rat model was made by restraint stress combined with ig rhubarb.After modeling,corresponding drugs were given at the dose,once a day for 14 days.TLR4 mRNA expression was observed through PCR method.Result： The expression of TLR4 in the model group（1.229 ± 0.172） was higher than that in the control group（1.109 ± 0.062）（P 0.01）.While the expression of TLR4 in the FZLZ decoction was similar to normal groups,there was no significant difference between the high（1.068 ± 0.056） and low（1.067 ± 0.071） dose group,pinaverium bromide group（1.205 ± 0.290） compared with the normal group TLR4 expression increased（P 0.01）.Conclusion： FZLZ decoction may maintain intestinal homeostasis by enhancing the whole body tolerance,and decreaseing the expression of TLR4 and immune response.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第15期218-221,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省中医药局资助项目(20111261)
关键词
附子理中汤
腹泻型
肠易激综合征
Fuzi Lizhong decoction
diarrhea type
irritable bowel syndrome
