摘要
目的初步探讨大鼠脑室出血后急性期脑损伤的病理特点。方法健康成年雄性SD大鼠38只按随机数字表法分为对照组和脑室出血组．分别采用立体定向技术向右侧脑室内注入生理盐水200μL或自体股动脉血200μL手术建模。术后24h观察大鼠体质量减轻情况，采用小动物Micro—CT扫描及大体标本脑室切面观察脑室铸型：采用干湿重法测定术后24h大鼠脑组织含水量；采用伊文思蓝染色法（24h时）和IgG免疫荧光染色法（48h时）观察大鼠血脑屏障损伤情况。结果术后24h时脑室出血组体质量减轻（5．29％±0．59％）明显高于对照组（3．36％±1．10％），差异有统计学意义沪〈0．05）。脑室出血组Micro．CT扫描及脑室切面可见脑室积血铸型并明显扩张。脑室出血组双侧大脑半球脑组织含水量（左侧：78．25％±0．29％；右侧：78．46％±0．21％）均明显高于对照组（左侧：77．64％±0．25％；右侧：77．91％±0．10％），差异有统计学意义（P〈0．05）。术后24h时脑室出血组伊文思蓝脑组织渗出量明显高于对照组，48h时脑室出血组侧脑室脉络丛免疫球蛋白IgG表达明显高于对照组。结论脑室出血后脑损伤除脑积水外还存在急性脑水肿、血脑屏障损伤等病理损害，其可能是脑室出血后除继发脑积水外急性脑损伤的重要环节。
Objective To investigate the pathological characteristics of acute brain injury in rat models of intraventricular hemorrhage （IVH）. Methods Thirty-eight male Sprague-Dawley rats were randomly divided into control group and 1VH group; stereotactic technique was employed to perform a 200-μL injection of either saline （control group） or autologous blood （IVH group） into the right lateral ventricle and these rats were euthanized at different time points. The weight loss of the rats was observed at 24 h after injection. The morphological changes of the cerebral ventricle were investigated using Micro-CT and brain samples. The brain water content by wet-dry weighting method was used to evaluate brain edema after IVH. Finally, blood brain barrier （BBB） injury was investigated by Evans blue （EB） staining （24 h after injection） and IgG immunofluorescent tests （48 h after injection）. Results The weight loss in the IVH group was significantly more obvious than that in the control group 24 h after the injection （P〈0.05）. Ventricular dilatation and cast form of ventricle were noted at the IVH group. The brain water content of the IVH group significantly increased in bilateral cerebral hemispheres as compared with that of the control group （P〈0.05）. Evans blue exudation into the brain tissue and IgG expression in ventricle choroid plexus in the IVH group obviously increased as compared with those in the control group. Conclusion Besides hydrocephalus, acute brain injury after IVH might also accompany with acute ecephaledema and BBB damage, which are probably important pathologicalcharacteristics in acute brain injury after IVH.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2013年第7期665-669,共5页
Chinese Journal of Neuromedicine
基金
国家自然科学基金面上项目（81271281、81070929）
关键词
脑室出血
脑水肿
血脑屏障
Intraventricular hemorrhage
Encephaledema
Blood brain barrier
