摘要
目的研究体外大量扩增小鼠树突状细胞(DCs)的实验方法。方法用细胞因子[肿瘤坏死因子-α(TNF-α)、白介素-4(IL-4)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)]组合体外诱导培养DCs,混合淋巴细胞实验检测DCs的免疫刺激活性。结果培养6-8 d,细胞表面有大量毛刺状突起生长,即DCs;该DCs具有很强的刺激T淋巴细胞增殖的能力,且在T细胞与DCs比值为10∶1时最强。结论 TNF-α、IL-4、GM-CSF组合是诱导体外扩增DCs的较佳方法。
Objective To study the experiment method for largely expanding mice dendritic cells（DCs） in vitro.Methods The lymphokines combined with tumor necrosis factor-α（TNF-α）,interleukin-4（IL-4） and granulocyte-macrophage colonystimulating factor（GM-CSF） were adopted for inducing and cultivating DCs in vitro;the immunostimulatory activity of DCs was detected by the mixed lymphocyte reaction.Results The cell surface exhibited a large number of burr-lke process on 6-8 d of culture,i.e.,DCs;the cultured DCs had the ability to strongly immunostimulate the proliferation of T lymphocytes,which with the T cells/DCs ratio of 10：1 was strongest.Conclusion The combination of TNF-α,IL-4 and GM-CSF is a better method for largely expanding mice DCs in vitro.
出处
《现代医药卫生》
2013年第13期1972-1973,共2页
Journal of Modern Medicine & Health
