摘要
目的研究肺炎支原体(Mp)荚膜多糖(CPS)与树突状细胞(DC)特异性细胞间黏附分子-3-结合非整合素分子(DC-SIGN,CD209)的相互作用及其对未成熟DC(iDC)分泌IL-10和IL-12的影响。方法提取并纯化Mp的CPS和脂质相关膜蛋白(LAMPs),用间接免疫荧光检测CPS与DC-SIGN受体的作用;ELISA法检测CPS和LAMPs作用后iDC所分泌的IL-10,IL-12水平。结果间接免疫荧光可见Mp CPS可结合到DC表面,且DC-SIGN的特异性封闭抗体可阻断CPS与DC的结合;ELISA检测发现CPS与LAMPs共孵育可促进DC分泌IL-10(P<0.05),而IL-12的表达水平刺激前后无统计学的差异。结论 Mp的CPS可识别并结合DC的DC-SIGN受体,并促进未成熟DC分泌IL-10。
Objective To explore the interaction of Mycop/asma pneumoniae （ M. pneumoniae） capsular polysaccharide （CPS） with dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin （DC-SIGN） and its influence on the secretions of IL-10 and IL-12. Methods M. pneurnoniae CPS and lipid-associated membrane proteins （LAMPs） were extracted and purified, and the specific binding of CPS and DC-SIGN was detected using indirect immunofluorescence stai- ning. The expressions of IL-10 and IL-12 was tested by ELISA in immature DC after treated by CPS or/and LAMPs. Results Indirect immunofluorescence staining showed CPS could recognized and bind specifically to the DC, which could be blocked by the DC-SIGN-specific blocking antibody. ELISA revealed that the production of immunosuppressive cytokine IL-10 increased when immature DC were stimulated by CPS, and the effect was more obvious with the presence of LAMPs（ P 〈 0. 05）. But the production of IL-12 did not change significantly after stimulation of CPS or/and LAMPs. Conclusion The CPS of M. pneumoniae can recognize and bind to the DC-SIGN specifically and promote the immature DC to secret IL-10.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2013年第1期10-13,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81072418)
