摘要
[目的]探究提取紫花苜蓿中多糖的最佳工艺条件。[方法]采用热水浸提法提取紫花苜蓿中多糖,并通过单因素试验和正交试验研究了浸提时间、浸提温度、浸提固液比、浸提次数对提取紫花苜蓿中多糖得率的影响。[结果]紫花苜蓿中多糖的最佳提取工艺条件为浸提时间1 h、浸提温度95℃、固液比1∶30、浸提3次。在该最佳提取条件下,多糖的得率可达6.1%。[结论]为紫花苜蓿多糖的研究和生产提供了理论依据。
[ Objective ] The aim was to study the optimal extraction conditions of polysaccharide from Medicago sativa. [ Method ] The polysac- charide was extracted from Medicago sativa by using hot water extraction method. Effects of four factors like extraction time, extraction tempera- ture, solid-liquid ratio and extraction times on the extracting rate of polysaccharide extracted from Medicago sativa were studied through single-fac- tor experiment and orthogonal experiment. [ Result ] The optimal extraction conditions of polysaccharide from Medicago sativa were as followed ： the extraction time was 1 h ; the extraction temperature was 95 ℃ ; the solid-liquid ratio was 1 ： 30 ; and the extracting time was three times. Under the above conditions, the extracting rate of polysaccharide was up to 6.1%. [ Conclusion ] The research results provides theoretical basis for the study and production of polysaccharide from Medicago sativa.
出处
《安徽农业科学》
CAS
2013年第14期6302-6304,共3页
Journal of Anhui Agricultural Sciences
基金
青海省科技攻关项目(2005-N-103)
农业部公益行业(农业)专项(201203007)
关键词
苜蓿
多糖
提取
正交试验
测定
Medicago sativa
Polysaccharide
Extraction
Orthogonal test
Determination
