摘要
目的探讨11β-羟基类固醇脱氢酶1(11β-HSD1)基因多态性与2型糖尿病及其不同临床表型患者的关系。方法选择广州医学院第三附属医院和中山大学附属第三医院内分泌科新诊断为2型糖尿病患者345例,根据其空腹血糖和餐后血糖水平的不同,将2型糖尿病分为单纯空腹高血糖(110例)、单纯餐后高血糖(127例)和空腹并餐后高血糖(108例)三种不同临床表型,同时选择96名健康体查者作为对照组进行比较。采用改良碘化钠提取外周血白细胞DNA,应用套式PCR进行目的片段扩增,通过基因测序检测11β-HSD15′端-2940多态位点rs846910-G/A基因型及等位基因分布频率,比较2型糖尿病不同临床表型患者及对照组基因型和等位基因分布频率差异。结果 rs846910-A/G的AA、AG、GG基因型以GG较常见,AA较少见。单纯空腹高血糖者的AA、AG、GG基因型的频率分别为5.0%、16.0%和84.0%,单纯餐后高血糖者分别为4.0%、16.6%和80.0%,空腹并餐后高血糖者分别为4.7%、15.8%和79.2%,对照组分别为5.9%、32.9%和61.2%,四组之间比较差异有统计学意义(P<0.05),但三种不同临床表型者之间比较差异无统计学意义(P>0.05)。G和A等位基因分布频率主要以G等位基因为主。单纯空腹高血糖者的G和A等位基因分布频率分别为85.2%和14.8%,单纯餐后高血糖者分别为83.5%和16.5%,空腹并餐后高血糖者分别为85.4%和14.6%,对照组分别为75.9%和24.1%,四组之间比较差异有统计学意义(P<0.05),但三种不同临床表型者之间比较差异无统计学意义(P>0.05)。结论 2型糖尿病患者的11β-HSD1基因多态性与健康对照者分布不同,但在不同临床表型患者之间相似,11β-HSD1基因多态性可能与2型糖尿病发生有关。
Objective To observe the correlation between the 11β-HSD1 gene polymorphism and clinical phenotypes of type 2 diabetes. Methods A total of 96 controls and 345 patients with newly diagnosed type 2 diabetes at the Third Affiliated Hospital of Guangzhou Medical College and Third Affiliated Hospital of Sun Yat-sen University were enrolled in our study. The patients with type 2 diabetes were divided into 3 groups based on the level of fasting blood glucose and 2 h postprandial blood glucose. DNA samples were extracted by the modified sodium iodide extraction method for the nested PCR amplification of target fragments followed by sequencing to determine the 11β-HSD1 5′ end-2940 polymorphic loci rs846910-G / A genotype. Allele and genotype distribution frequencies were compared among different groups. Results GG genotype was common in the rs846910-G / A, however AA genotype was rare. The AA, AG, GG genotype frequencies in isolated fasting hyperglycemia （IFH） were 5.0%, 16.0% and 84.0% , respectively. The AA, AG, GG genotype frequencies in isolated postprandial hyperglycemia （IPH） were 4.0% , 16.6% and 80.0% , respectively. The AA, AG, GG genotype frequencies in combinedhyperglycemia （CH） were 4.7% , 15.8% and 79.2% , respectively. The AA, AG, GG genotype frequencies in control group were 5.9%, 32.9% and 61.2%, respectively. These frequences were significantly different among the four groups （P〈0.05）, but no statistically significant difference between the three different clinical phenotypes （P〈0.05）. The G allele was common in the rs846910-A / G, however the A allele was rare. The A and G alleles frequencies in IFH were 85.2% and 14.8%, respectively. The A and G alleles frequencies in IPH were 83.5% and 16.5% , respectively. The A and G alleles frequencies in CH were 85.4% and 14.6% , respectively. The A and G alleles frequencies in the control group were 75.9% and 24.1% , respectively. They were significantly different among the four groups （P〈0.05）, but no statistically significant di
出处
《热带医学杂志》
CAS
2013年第6期745-748,共4页
Journal of Tropical Medicine
基金
广州医学院第三附属医院青年科研支持计划项目(2011Y12)
