摘要
目的建立红松松塔多糖含量测定方法。方法采用改良差示酚硫法测定红松松塔多糖含量。结果多糖溶液沸水浴30min水解完全,显色液预配,用量为5.0mL;显色后的待测液在2h内稳定;测定波长λmax=490nm;线性范围为3.57～21.42μg·mL-1,r=0.999;回收率为98.3%,RSD为1.06%。结论该方法可用于红松松塔多糖的含量测定,含杂质的样品不必进行繁琐的预分离纯化,方法简便、准确,重复性好。
Objective To establish a method to determine the contents of polysaccharides from pine cone of Pinus koraiensis or from preparations. Methods The polysaccharides from pine cone were determined by an.improved-differential phenol sulfuric method. Result Polysaccharides were hydrolyzed to monosaccharide in boiling water bath for 30 min. 5.0 mL of the color liquid was used. The color was stable within 2 h;the calibration curve was linear in the range from 3.57 to 21.42μg·mL^-1 ,with r 0. 999. The average recovery was 98.3% with RSD 1.06%. Conclusions The irnproved-diferential phenol sulfuric method is simple, precision and repeatable for the determination of polysaccharides from pine cone of Pinus koraiensis and from preparations of pharmaceutics that contain impurities.
出处
《西北药学杂志》
CAS
2013年第4期340-342,共3页
Northwest Pharmaceutical Journal
关键词
多糖
苯酚硫酸法
改良差示酚硫法
含量测定
红松松塔
polysaccharides
phenol sulfuric method
improved-diferential phenol sulfuric method
content analysis
pine cone of Pinus koraiensis
