摘要
用交叉引物恒温扩增技术(Cross priming amplification,CPA)和核酸试纸条检测方法(Nucleic acid test strip detection methord)快速检测霍乱弧菌。根据霍乱弧菌的MDH基因序列设计特异性的引物和探针,通过对反应体系与反应条件进行优化,确立最佳反应体系,并将CPA方法与PCR法进行比较与评价。对霍乱弧菌的检测具有高度特异性,对霍乱弧菌的检出极限达到了6×102CFU/mL,高于PCR,而且具有较好的稳定性。交叉引物恒温扩增技术(Cross priming amplification,CPA)-核酸试纸条检测方法(Nucleic acid test strip detection methord)是一种特异、灵敏、快速、简便的霍乱弧菌的检测方法。
It was to estabilish a cross priming amplification and nucleic acid test strip based rapid detection method of Vibrio cholerae. Specific primers and probes according to the the MDH gene sequence of the Vibrio cholerae were designed, and via optimizing the reaction system and the reaction conditions to establish the optimal reaction system, the CPA method with the PCR method were compared and evaluated. Result showed that the method was highly specific for the detection of Vibrio cholerae, the detection hmit reached 6 × 10^2 CFU/mL, higher than that of the PCR, and has satisfactorg stability. The CPA-nucleic acid test strip detection method established in this study is a specific, sensitive, rapid and easy detection method of Vibrio cholerae.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第7期167-171,共5页
Biotechnology Bulletin
