摘要
目的探讨经热处理及酶裂解的肠球菌faecalis FK-23菌株（简称LFK）和经热处理的肠球菌faeciumsp．TN-3菌株（简称TN）对实验性变应性鼻炎（allergic rhinitis，AR）的抗变态反应作用。方法BALB／c小鼠24只完全随机分为4组：阳性对照组、LFK组、TN组、阴性对照组。采用卵清蛋白（ovalbumin，OVA）腹腔注射及鼻腔激发建立AR小鼠模型，阴性对照组以生理盐水代替OVA进行腹腔注射及鼻腔激发。LFK组和TN组分别给予相应的益生菌制品对小鼠进行经口灌胃，每次0．5ml（60mg），每日1次，共计42d；阳性和阴性对照组用生理盐水灌胃处理。观察经灌胃干预后第21、27、35天小鼠搔鼻和喷嚏次数。第42天取小鼠鼻部组织进行HE染色，观察鼻黏膜嗜酸粒细胞的浸润程度。采用酶联免疫吸附试验检测小鼠血清和脾细胞培养液中^y干扰素（interferon，IFN）、白细胞介素（interleukin，IL）4和OVA特异性IgE水平。结果①在27d，LFK组小鼠的搔鼻次数与阳性对照组相比明显减少，差异有统计学意义（t=2．95，P=0．028）；在35d，LFK组、TN组小鼠的搔鼻次数（t值分别为3．75和3．06，P值分别为0．005和0．011）和喷嚏次数（t值分别为2．56和3．35，P值分别为0．038和0．01）均明显减少，与阳性对照组相比差异有统计学意义。②在42d，小鼠鼻黏膜组织切片HE染色显示，LFK组、TN组的嗜酸粒细胞计数与阳性对照组相比均明显减少（￡值分别为3。44和2．97，P值分别为0．014和0．025），但两组的嗜酸粒细胞计数仍明显高于阴性对照组（t值分别为2．54和3．39，P值分别为0．044和0．015）。③在42d，LFK组小鼠的血清IL_4和OVA特异性IgE水平、脾细胞培养液中IFN一1和IL-4水平与阳性对照组相比，差异均无统计学意义（P值均〉0．05）。TN组小鼠血清IFN一1水平为（27．07±3．83）pg／ml，明显高于LFK组的（14．834-0．99）pg�
Objective The aim of this study was to investigate the anti-allergic effects of lysozyme/ heat-treated Enterococcusfaecalis FK-23 （LFK） and heat-treated Enterococcus faecium sp. TN-3 （TN） on experimental allergic rhinitis （AR）. Methods A total of twenty-four BALB/c mice were divided into four groups randomly ： （ 1 ） positive control group, （2） LFK-fed group, （ 3 ） TN-fed group, and （4） negative control group. To establish the murine AR model, intraperitoneal injection and nasal drip with ovalbumin （OVA） were performed. Saline was used instead of OVA for negative control. Probiotic preparations of LFK and TN were orally administrated for 42 days [ 60 mg （0. 5 ml）/d] in LFK-fed and TN-fed mice, respectively. The positive and negative control mice received saline orally for 42 days. Nasal rubbing and sneezing were monitored on d 21, d 27, and d 35. After the final challenge, mice were sacrificed on d 42, and eosinophilic infiltration into the nasal mucosa was quantified （H＆E stain）. IFN-＇,/, IL-4 and OVA- specific IgE levels in the sera and splenocyte culture supernatants were determined by ELISA kits. Results Nasal rubbing of LFK-fed mice was significantly reduced compared to the positive control group on day 27 （ t = 2. 95, P = 0. 028）. Both in the LFK-fed and TN-fed mice, nasal rubbing （t value was 3.75 and 3.06, P value was 0. 005 and 0. 011, respectively） and sneezing （t value was 2. 56 and 3.35, P value was 0. 038 and 0.01, respectively） were significantly decreased compared to the positive control group on d 35. The H＆E strain section of nasal tissue showed that eosinophil influx into the nasal mucosa decreased significantly both in the LFK-fed and TN-fed mice compared to the positive control group on day 42 （t value was 3.44 and 2. 97, P value was 0. 014 and 0. 025, respectively） ; however, the LFK-fed mice and TN-fed mice had significant eosinophil influx into the nasal mucosa than that in the negative control group （ t value was 2. 54 and 3.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2013年第7期555-562,共8页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
基金项目：江苏省国际科技合作计划（BZ2011045）
江苏高校优势学科建设工程（PAPD2010-2013）
江苏省“科教兴卫工程”医学重点学科（XK200719）、重点人才（RC2007065,RC2011071）
南京医科大学科技发展基金重点项目（2012NJMU091）
