摘要
目的探讨茶多酚(TP)对缺氧诱导的大鼠肺高血压(PH)的作用及可能机制。方法 24只SD大鼠随机均分为正常对照组(C)、缺氧组(H)和TP组。缺氧前TP组大鼠予TP灌胃(200mg.kg-1.d-1),C和H组大鼠接受等体积生理盐水灌胃,随后TP和H组大鼠置于常压间断缺氧舱(8h/d,6天/周)4周。4周后比较各组的右心室收缩压(RVSP)、平均肺动脉压(mPAP)、右心室肥厚指数(RVHI)及肺小动脉管壁厚度,比较肺动脉平滑肌细胞(PASMCs)凋亡及肺组织XIAP mRNA表达水平。结果 H组大鼠的RVSP、mPAP、RVHI、肺小动脉管壁厚度均比C组显著升高(P<0.01),且PASMCs凋亡明显减少(P<0.01),肺组织XIAP mRNA表达显著升高(P<0.01)。与H组相比,TP组RVSP、mPAP及RVHI均明显降低,肺小动脉管壁增厚程度减轻,而PASMCs凋亡增加(P<0.05),肺组织XIAP mRNA表达降低(P<0.05)。结论 TP可改善缺氧诱导的大鼠PH,其机制可能与抑制肺组织XIAP mRNA表达水平促进PASMCs凋亡有关。
Objective To investigate the effects of tea polyphenols(TP) on hypoxia induced pulmonary hypertension(PH) in rats.Methods Twenty-four SD rats were randomly divided into three groups:normal control group(C),hypoxia group(H) and TP group.Before the hypoxia state,The TP group were given TP(200 mg·kg-1·d-1),The C and H groups were given normal saline at the same dosage,then TP group kept in atmospheric pressure with intermittent hypoxia together with the H group(8 h/d,6 d/w)in 4 weeks.After 4 weeks,RVSP,mPAP,RVHI,wall thickness of small pulmonary artery were compared.Apoptosis of PASMCs and the mRNA expression of XIAP were also detected.Results Compared with C group,there were rise in RVSP,mPAP,RVHI,wall thickness of small pulmonary artery in H group(P 0.01),decrease in PASMCs muscle cell apoptosis(P0.01) and increase in XIAP mRNA expression(P0.01).There were decrease in RVSP,mPAP,RVHI,wall thickness of small pulmonary artery in TP group compare with H group,increase in PASMCs apoptosis(P0.05) and reduction in XIAP mRNA expression(P0.05) in TP group.Conclusion TP ameliorate hypoxia induced pulmonary hypertension in rats,its mechanism may be related to inhibit XIAP mRNA expression level then promote the apoptosis of PASMCs.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第19期2243-2245,共3页
Chongqing medicine
基金
广西自然科学基金资助项目(2010GXNSFB013074)
关键词
高血压
肺性
细胞凋亡
X相关凋亡抑制蛋白
茶多酚
hypertension,pulmonary
apoptosis
X-linked inhibitor of apoptosis protein
tea polyphenols