摘要
OBJECTIVE： This work aims to observe the effects of electroacupuncture on brain-derived neuro- trophic factor （BDNF） mRNA expression in mouse hippocampus following cerebral ischemia-reperfu- sion injury. METHODS： The models of mouse cerebral isch- emia-reperfusion injury were established. A total of 96 healthy mice were randomly assigned into 4 groups, namely, the sham surgery, model, mod- el ＋ electroacupuncture, and mode ＋ hydergine groups. Mice in the model ＋ electroacupuncturegroup were treated through electroacupuncture at the Shenshu （BL 23）, Geshu （BL 17）, and Baihui （GV 20） acupoints. Mice in the model＋hydergine group were intragastrically administered with hy- dergine （0.77 mg/kg-1.day-l）. The levels of BDNF mRNA expressions in the hippocampus were ana- lyzed through a semi-quantitative reverse transcrip- tion-polymerase chain reaction assay on days 1 and 7 after the surgeries. RESULTS： BDNF mRNA expressions in the mouse hippocampus of the model group on days 1 and 7 after the surgery were higher than those of the sham surgery group （both P〈0.01）. On days 1 and 7 of the electroacupuncture treatment, BDNF mRNA expression in the mouse hippocampus of the mod- el ＋ electroacupuncture group was significantly ele- vated compared with the model group （both P〈 0.01） or the model ＋ hydergine group （both P〈 0.01）. On days 1 and 7 of the hydergine treatment, BDNF mRNA expression in the mouse hippocam- pus of the model ＋ hydergine group tended to in- crease compared with the model group; however, statistical significance was not achieved （both P〉 0.05）. CONCLUSION： Electroacupuncture treatment en- hances endogenous BDNF expression, which may improve the survival environment for intracerebral neurons and inhibit the apoptosis of hippocampal cells.
OBJECTIVE:This work aims to observe the effects of electroacupuncture on brain-derived neurotrophic factor(BDNF) mRNA expression in mouse hippocampus following cerebral ischemia-reperfusion injury.METHODS:The models of mouse cerebral ischemia-reperfusion injury were established.A total of 96 healthy mice were randomly assigned into 4 groups,namely,the sham surgery,model,model + electroacupuncture,and mode + hydergine groups.Mice in the model + electroacupuncture group were treated through electroacupuncture at the Shenshu(BL 23),Geshu(BL 17),and Baihui(GV 20) acupoints.Mice in the model+hydergine group were intragastrically administered with hydergine(0.77 mg/kg-1 · day-1).The levels of BDNF mRNA expressions in the hippocampus were analyzed through a semi-quantitative reverse transcription-polymerase chain reaction assay on days 1 and 7 after the surgeries.RESULTS:BDNF mRNA expressions in the mouse hippocampus of the model group on days 1 and 7 after the surgery were higher than those of the sham surgery group(both P<0.01).On days 1 and 7 of the electroacupuncture treatment,BDNF mRNA expression in the mouse hippocampus of the model + electroacupuncture group was significantly elevated compared with the model group(both P< 0.01) or the model + hydergine group(both P< 0.01).On days 1 and 7 of the hydergine treatment,BDNF mRNA expression in the mouse hippocampus of the model + hydergine group tended to increase compared with the model group;however,statistical significance was not achieved(both P> 0.05).CONCLUSION:Electroacupuncture treatment enhances endogenous BDNF expression,which may improve the survival environment for intracerebral neurons and inhibit the apoptosis of hippocampal cells.
基金
Supported by Department of Science & Technology of He-bei Province (No. 06276102D-31)
Department of Health of Hebei Province (No. 2005156)
Training Program for Backbone of Scientific Research Talents of Hebei Medical University (2007)
